UM
4 '-Hydroxywogonin suppresses lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and acute lung injury mice
Fan, Chao; Wu, Le-Hao; Zhang, Gu-Fang; Xu, Fangfang; Zhang, Shuo; Zhang, Xiuli; Sun, Lei; Yu, Yang; Zhang, Yan; Ye, Richard D.
2017-08-08
Source PublicationPLOS ONE
ISSN1932-6203
Volume12Issue:8
Abstract4'-Hydroxywogonin (4'-HW), a flavonoid, has been isolated from various plants and shown to inhibit NO production in macrophages. However, the molecular mechanisms and its in vivo activity have not been determined. Our study aimed to investigate the mechanisms underlying the anti-inflammatory effects of 4'-HW in vitro and in vivo. We showed that 4'-HW potently reduced the expression levels of COX-2 and iNOS as well as their products, prostaglandin E2 (PGE2) and nitric oxide (NO) respectively, in LPS-stimulated RAW 264.7 macrophages. 4'-HW also suppressed LPS-induced pro-inflammatory cytokines at mRNA and protein levels. Moreover, 4'-HW blocked the interaction of TAK1 and TAB1 in LPS-stimulated RAW 264.7 macrophages, resulting in an inhibition of the TAK1/IKK/NF-kappa B signaling pathway. Furthermore, 4'-HW also reduced the phosphorylation of MAPKs and PI3/Akt signaling pathways in LPS-stimulated RAW 264.7 macrophages. 4'-HW was also significantly decreased the intracellular reactive oxygen species (ROS) level. The effect of 4'-HW was confirmed in vivo. 4'-HW exhibited potent protective effect against LPS-induced ALI in mice. These findings indicate that 4'-HW suppresses the LPS-induced response in vitro and in vivo. It is likely that the inhibition of the TAK1/IKK/NF-kappa B, MAPKs and PI3/AKT signaling pathways contribute to the anti-inflammatory effects of 4'-HW. Our study suggests that 4'-HW may be an important functional constituent in the plants and has the potential value to be developed as a novel anti-inflammatory agent.
DOI10.1371/journal.pone.0181191
URLView the original
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000407144700004
PublisherPUBLIC LIBRARY SCIENCE
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Fan, Chao,Wu, Le-Hao,Zhang, Gu-Fang,et al. 4 '-Hydroxywogonin suppresses lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and acute lung injury mice[J]. PLOS ONE,2017,12(8).
APA Fan, Chao.,Wu, Le-Hao.,Zhang, Gu-Fang.,Xu, Fangfang.,Zhang, Shuo.,...&Ye, Richard D..(2017).4 '-Hydroxywogonin suppresses lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and acute lung injury mice.PLOS ONE,12(8).
MLA Fan, Chao,et al."4 '-Hydroxywogonin suppresses lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and acute lung injury mice".PLOS ONE 12.8(2017).
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Fan, Chao]'s Articles
[Wu, Le-Hao]'s Articles
[Zhang, Gu-Fang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Fan, Chao]'s Articles
[Wu, Le-Hao]'s Articles
[Zhang, Gu-Fang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Fan, Chao]'s Articles
[Wu, Le-Hao]'s Articles
[Zhang, Gu-Fang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.