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Baicalein prevents 6-OHDA/ascorbic acid-induced calcium-dependent dopaminergic neuronal cell death
Wang, Sheng-Fang1; Liu, Liang-Feng2,3,4; Wu, Ming-Yue1; Cai, Cui-Zan1; Su, Huanxing1; Tan, Jieqiong5; Lu, Jia-Hong1; Li, Min2,3
2017-08-21
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume7
Abstract

6-OHDA plus ascorbic acid (AA) has long been used to induce Parkinson's disease in rodents, while only 6-OHDA is commonly used to induce cell damage in cellular PD models. AA was believed to act as an anti-oxidant to prevent the degradation of 6-OHDA; however, some studies suggested that AA dramatically enhanced the selectivity and toxicity of 6-OHDA. To understand the mechanisms by which 6-OHDA/AA induces cell death, we established a 6-OHDA/AA cell toxicity model in human dopaminergic neuroblastoma SH-SY5Y cells. We confirmed that the toxicity of 6-OHDA was dramatically increased in the presence of AA, and the toxicity can be prevented by a flavonoid, baicalein. Mechanistically, our research reveals that 6-OHDA/AA induces cell death mainly through the interruption of intracellular calcium homeostasis, which leads to calpain activation and mitochondrial damage. Baicalein prevents 6-OHDA/AA-induced intracellular calcium elevation as well as consequent mitochondria damage. Taken together, our study confirms that 6-OHDA/AA is a more sensitive model for inducing neuronal lesion in vitro and reveals the central role of intracellular calcium in 6-OHDA/AA-induced cell death. Our studies further show that baicalein prevents 6-OHDA/AA-induced cell death by inhibiting intracellular calcium elevation.

DOI10.1038/s41598-017-07142-7
URLView the original
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000408103900006
PublisherNATURE PUBLISHING GROUP
The Source to ArticleWOS
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao SAR, China
2.School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong
3.Mr. and Mrs. Ko Chi Ming Centre for Parkinson’s Disease Research, Hong Kong Baptist University, Kowloon, Hong Kong
4.Limin Pharmaceutical Factory, Livzon Pharmaceutical Group Inc. Shaoguan, Guangdong, China
5.State Key Laboratory of Medical Genetics, Xiangya Medical School, Central South University, Changsha, Hunan, China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wang, Sheng-Fang,Liu, Liang-Feng,Wu, Ming-Yue,et al. Baicalein prevents 6-OHDA/ascorbic acid-induced calcium-dependent dopaminergic neuronal cell death[J]. SCIENTIFIC REPORTS,2017,7.
APA Wang, Sheng-Fang.,Liu, Liang-Feng.,Wu, Ming-Yue.,Cai, Cui-Zan.,Su, Huanxing.,...&Li, Min.(2017).Baicalein prevents 6-OHDA/ascorbic acid-induced calcium-dependent dopaminergic neuronal cell death.SCIENTIFIC REPORTS,7.
MLA Wang, Sheng-Fang,et al."Baicalein prevents 6-OHDA/ascorbic acid-induced calcium-dependent dopaminergic neuronal cell death".SCIENTIFIC REPORTS 7(2017).
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