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Drug screening of cancer cell lines and human primary tumors using droplet microfluidics
Wong, Ada Hang-Heng1; Li, Haoran2; Jia, Yanwei2; Mak, Pui-In2,3; da Silva Martins, Rui Paulo2,3,4; Liu, Yan5; Vong, Chi Man5; Wong, Hang Cheong6; Wong, Pak Kin6; Wang, Haitao1; Sun, Heng1; Deng, Chu-Xia1
2017-08-22
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume7Pages:9109
Abstract

Precision Medicine in Oncology requires tailoring of therapeutic strategies to individual cancer patients. Due to the limited quantity of tumor samples, this proves to be difficult, especially for early stage cancer patients whose tumors are small. In this study, we exploited a 2.4 x 2.4 centimeters polydimethylsiloxane (PDMS) based microfluidic chip which employed droplet microfluidics to conduct drug screens against suspended and adherent cancer cell lines, as well as cells dissociated from primary tumor of human patients. Single cells were dispersed in aqueous droplets and imaged within 24 hours of drug treatment to assess cell viability by ethidium homodimer 1 staining. Our results showed that 5 conditions could be screened for every 80,000 cells in one channel on our chip under current circumstances. Additionally, screening conditions have been adapted to both suspended and adherent cancer cells, giving versatility to potentially all types of cancers. Hence, this study provides a powerful tool for rapid, low-input drug screening of primary cancers within 24 hours after tumor resection from cancer patients. This paves the way for further technological advancement to cutting down sample size and increasing drug screening throughput in advent to personalized cancer therapy.

DOIhttp://doi.org/10.1038/s41598-017-08831-z
URLView the original
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000408282600001
PublisherNATURE PUBLISHING GROUP
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:16   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
INSTITUTE OF MICROELECTRONICS
DEPARTMENT OF ELECTRICAL AND COMPUTER ENGINEERING
DEPARTMENT OF ELECTROMECHANICAL ENGINEERING
DEPARTMENT OF COMPUTER AND INFORMATION SCIENCE
Corresponding AuthorDeng, Chu-Xia
Affiliation1.Cancer Centre, Faculty of Health Sciences, University of Macau, Macau, China
2.State-Key Laboratory of Analog and Mixed-Signal VLSI (AMSV), University of Macau, Macau, China
3.Department of Electrical and Computer Engineering, Faculty of Science and Technology, University of Macau, Macau, China
4.Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal
5.Department of Computer and Information Science, Faculty of Science and Technology, University of Macau, Macau, China
6.Department of Electromechanical Engineering, Faculty of Science and Technology, University of Macau, Macau, China
First Author AffilicationFaculty of Health Sciences
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Wong, Ada Hang-Heng,Li, Haoran,Jia, Yanwei,et al. Drug screening of cancer cell lines and human primary tumors using droplet microfluidics[J]. SCIENTIFIC REPORTS,2017,7:9109.
APA Wong, Ada Hang-Heng.,Li, Haoran.,Jia, Yanwei.,Mak, Pui-In.,da Silva Martins, Rui Paulo.,...&Deng, Chu-Xia.(2017).Drug screening of cancer cell lines and human primary tumors using droplet microfluidics.SCIENTIFIC REPORTS,7,9109.
MLA Wong, Ada Hang-Heng,et al."Drug screening of cancer cell lines and human primary tumors using droplet microfluidics".SCIENTIFIC REPORTS 7(2017):9109.
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