UM
Identification of candidate genes involved in isoquinoline alkaloids biosynthesis in Dactylicapnos scandens by transcriptome analysis
He, Si Mei; Song, Wan Ling; Cong, Kun; Wang, Xiao; Dong, Yang; Cai, Jing; Zhang, Jia Jin; Zhang, Guang Hui; Yang, Jian Li; Yang, Sheng Chao; Fan, Wei
2017-08-22
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume7
AbstractDactylicapnos scandens (D. Don) Hutch (Papaveraceae) is a well-known traditional Chinese herb used for treatment of hypertension, inflammation, bleeding and pain for centuries. Although the major bioactive components in this herb are considered as isoquinoline alkaloids (IQAs), little is known about molecular basis of their biosynthesis. Here, we carried out transcriptomic analysis of roots, leaves and stems of D. scandens, and obtained a total of 96,741 unigenes. Based on gene expression and phylogenetic relationship, we proposed the biosynthetic pathways of isocorydine, corydine, glaucine and sinomenine, and identified 67 unigenes encoding enzymes potentially involved in biosynthesis of IQAs in D. scandens. High performance liquid chromatography analysis demonstrated that while isocorydine is the most abundant IQA in D. scandens, the last O-methylation biosynthesis step remains unclear. Further enzyme activity assay, for the first time, characterized a gene encoding O-methyltransferase (DsOMT), which catalyzes O-methylation at C7 of (S)-corytuberine to form isocorydine. We also identified candidate transcription factor genes belonging to WRKY and bHLH families that may be involved in the regulation of IQAs biosynthesis. Taken together, we first provided valuable genetic information for D. scandens, shedding light on candidate genes involved in IQA biosynthesis, which will be critical for further gene functional characterization.
DOI10.1038/s41598-017-08672-w
URLView the original
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000408282900003
PublisherNATURE PUBLISHING GROUP
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:11   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
He, Si Mei,Song, Wan Ling,Cong, Kun,et al. Identification of candidate genes involved in isoquinoline alkaloids biosynthesis in Dactylicapnos scandens by transcriptome analysis[J]. SCIENTIFIC REPORTS,2017,7.
APA He, Si Mei.,Song, Wan Ling.,Cong, Kun.,Wang, Xiao.,Dong, Yang.,...&Fan, Wei.(2017).Identification of candidate genes involved in isoquinoline alkaloids biosynthesis in Dactylicapnos scandens by transcriptome analysis.SCIENTIFIC REPORTS,7.
MLA He, Si Mei,et al."Identification of candidate genes involved in isoquinoline alkaloids biosynthesis in Dactylicapnos scandens by transcriptome analysis".SCIENTIFIC REPORTS 7(2017).
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[He, Si Mei]'s Articles
[Song, Wan Ling]'s Articles
[Cong, Kun]'s Articles
Baidu academic
Similar articles in Baidu academic
[He, Si Mei]'s Articles
[Song, Wan Ling]'s Articles
[Cong, Kun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[He, Si Mei]'s Articles
[Song, Wan Ling]'s Articles
[Cong, Kun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.