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Digital microfluidic system for LAMP-based detection of Trypanosoma brucei using molecular beacon probes
Liang Wan1; Tianlan Chen1,2; Jie Gao1; Cheng Dong1,2; Yanwei Jia1; Pui-In Mak1; Rui P. Martins1,2,3
2016
Conference NameLab on a Chip International Symposium: Droplet-based Microfluidics
Source PublicationLab on a Chip International Symposium: Droplet-based Microfluidics
Conference DateNovember 2016.
Conference PlaceHang Zhou, China,
Abstract

Digital microfluidics (DMF) is an emerging technology in the microfluidic field to manipulate individual droplet on an electrodes array by electro-wetting forces.1 Its feature of electric driving and miniature footprint has promised it as a perfect technology for point-of-care (POC) diagnostics.2 In molecular diagnostics, Loopmediated isothermal amplification (LAMP) works at a constant temperature,3 significantly simplifies the requirement on reaction equipment. However, the widely accepted DNA detection using a double-stranded DNA dye after LAMP is less specific, given the possibility of primer-dimer formation.

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Fulltext Access
Document TypeConference paper
CollectionFaculty of Science and Technology
INSTITUTE OF MICROELECTRONICS
DEPARTMENT OF ELECTRICAL AND COMPUTER ENGINEERING
Affiliation1.State-Key Laboratory of Analog and Mixed-Signal VLSI, University of Macau, Macao
2.Department of ECE, Faculty of Science and Technology, University of Macau, Macao
3.On Leave from Instituto Superior Técnico, Universidade de Lisboa, Portugal
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Liang Wan,Tianlan Chen,Jie Gao,et al. Digital microfluidic system for LAMP-based detection of Trypanosoma brucei using molecular beacon probes[C],2016.
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