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Synergistic effects of Chuanxiong-Chishao herb-pair on promoting angiogenesis at network pharmacological and pharmacodynamic levels
Wang Yan1,2,3; Guo Gang4; Yang Bin-rui3; Xin Qi-qi2; Liao Qi-wen3; Lee Simon Ming-Yuen3; Hu Yuan-jia3; Chen Ke-jj2; Cong Wei-hong2
2017-09
Source PublicationCHINESE JOURNAL OF INTEGRATIVE MEDICINE
ISSN1672-0415
Volume23Issue:9Pages:654-662
Abstract

Objective: To investigate the synergistic effects of Chuanxiong-Chishao herb-pair (CCHP) on : To investigate the synergistic effects of Chuanxiong-Chishao herb-pair (CCHP) on promoting angiogenesis promoting angiogenesis in silico in silico and in vivo in vivo. Methods: The mechanisms of action of an herb-pair, Chuanxiong- : The mechanisms of action of an herb-pair, ChuanxiongChishao, were investigated using the network pharmacological and pharmacodynamic strategies involving Chishao, were investigated using the network pharmacological and pharmacodynamic strategies involving computational drug target prediction and network analysis, and experimental validation. A set of network computational drug target prediction and network analysis, and experimental validation. A set of network pharmacology methods were created to study the herbs in the context of targets and diseases networks, pharmacology methods were created to study the herbs in the context of targets and diseases networks, including prediction of target profiles and pharmacological actions of main active compounds in Chuanxiong including prediction of target profiles and pharmacological actions of main active compounds in Chuanxiong and Chishao. Furthermore, the therapeutic effects and putative molecular mechanisms of Chuanxiong-Chishao and Chishao. Furthermore, the therapeutic effects and putative molecular mechanisms of Chuanxiong-Chishao actions were experimentally validated in a chemical-induced vascular insuf actions were experimentally validated in a chemical-induced vascular insuffi ciency model of transgenic zebra ciency model of transgenic zebrafi sh in vivo in vivo. The mRNA expression of the predicted targets were further analyzed by real-time polymerase chain . The mRNA expression of the predicted targets were further analyzed by real-time polymerase chain reaction (RT-PCR). reaction (RT-PCR). Results Results: The computational prediction results found that the compounds in Chuanxiong : The computational prediction results found that the compounds in Chuanxiong have antithrombotic, antihypertensive, antiarrhythmic, and antiatherosclerotic activities, which were closely have antithrombotic, antihypertensive, antiarrhythmic, and antiatherosclerotic activities, which were closely related to protecting against hypoxic-ischemic encephalopathy, ischemic stroke, myocardial infarction and heart related to protecting against hypoxic-ischemic encephalopathy, ischemic stroke, myocardial infarction and heart failure. In addition, compounds in Chishao were found to participate in anti-in failure. In addition, compounds in Chishao were found to participate in anti-infl ammatory effect and analgesics. ammatory effect and analgesics. Particularly, estrogen receptor Particularly, estrogen receptor α (ESRα) and hypoxia-inducible factor 1- ) and hypoxia-inducible factor 1-α (HIF-1α) were the most important ) were the most important potential protein targets in the predicted results. potential protein targets in the predicted results. In vivo In vivo experimental validation showed that post-treatment of experimental validation showed that post-treatment of tetramethylpyrazine hydrochloride (TMP•HCl) and paeoniflorin (PF) promoted the regeneration of new blood tetramethylpyrazine hydrochloride (TMP•HCl) and paeoniflorin (PF) promoted the regeneration of new blood vessels in zebra vessels in zebrafi sh involving up-regulating ESR sh involving up-regulating ESRα mRNA expression. Co-treatment of TMP•HCl and PF could mRNA expression. Co-treatment of TMP•HCl and PF could enhance the vessel sprouting in chemical-induced vascular insuf enhance the vessel sprouting in chemical-induced vascular insuffi ciency zebra ciency zebrafi sh at the optimal compatibility sh at the optimal compatibility proportion of PF 10 proportion of PF 10 μmol/L with TMP•HCl 1 mol/L with TMP•HCl 1 μmol/L. Conclusions Conclusions: The network pharmacological strategies : The network pharmacological strategies combining drug target prediction and network analysis identified some putative targets of CCHP. Moreover, combining drug target prediction and network analysis identified some putative targets of CCHP. Moreover, the transgenic zebra the transgenic zebrafi sh experiments demonstrated that the Chuanxiong-Chishao combination synergistically sh experiments demonstrated that the Chuanxiong-Chishao combination synergistically promoted angiogenic activity, probably involving ESRα signaling pathway.

KeywordTetramethylpyrazine Paeoniflorin Angiogenesis Network Pharmacology
DOI10.1007/s11655-017-2408-x
URLView the original
Indexed BySCI
Language英语
WOS Research AreaIntegrative & Complementary Medicine
WOS SubjectIntegrative & Complementary Medicine
WOS IDWOS:000408880600003
PublisherSPRINGER
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:10   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorChen Ke-jj
Affiliation1.Clinical Medical College,Beijing University of Chinese Medicine,Beijing,China
2.Laboratory of Cardiovascular Diseases, Xiyuan Hospital China Academy of Chinese Medical Sciences,Beijing,China
3.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences,University of Macau,Macao SAR,China
4.Department of Acupuncture and Moxibustion,Qilu Hospital Affiliated to Shandong University,Jinan,China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wang Yan,Guo Gang,Yang Bin-rui,et al. Synergistic effects of Chuanxiong-Chishao herb-pair on promoting angiogenesis at network pharmacological and pharmacodynamic levels[J]. CHINESE JOURNAL OF INTEGRATIVE MEDICINE,2017,23(9):654-662.
APA Wang Yan.,Guo Gang.,Yang Bin-rui.,Xin Qi-qi.,Liao Qi-wen.,...&Cong Wei-hong.(2017).Synergistic effects of Chuanxiong-Chishao herb-pair on promoting angiogenesis at network pharmacological and pharmacodynamic levels.CHINESE JOURNAL OF INTEGRATIVE MEDICINE,23(9),654-662.
MLA Wang Yan,et al."Synergistic effects of Chuanxiong-Chishao herb-pair on promoting angiogenesis at network pharmacological and pharmacodynamic levels".CHINESE JOURNAL OF INTEGRATIVE MEDICINE 23.9(2017):654-662.
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