UM
Isolation, identification and characteristics of pyrene degrading bacteria Ochrobactrum sp.
Xie W.1; Lin A.1; Yang X.1; Wang F.3; Shim H.2
2011-05-01
Source PublicationBeijing Huagong Daxue Xuebao (Ziran Kexueban)/Journal of Beijing University of Chemical Technology (Natural Science Edition)
ISSN16714628
Volume38Issue:3Pages:76-80
AbstractA pyrene-degrading bacterium strain named PW was isolated from the soils contaminated with polycyclic aromatic hydrocarbons (PAHs) in Beijing Coking Plant using an enrichment culture. It was identified according to its morphology and molecular biology analysis. This bacterium belongs to the strain Ochrobactrum sp. This strain was domesticated for a period of time by a large dose method, and then shake flask experiments were used to study the influence of environmental conditions on the degradation extent of pyrene. The results showed that after domestication the degradation extent increased from 62.3% to 92.7% on the 5th day. The bacterium strain PW could degrade pyrene at temperatures ranging from 20 to 40°C, and the degradation extent at 30°C was the highest. The degradation extents were above 45% in cultural mediums at a wide range of pH from 5 to 10. The results also indicated that the pyrene degradation could be affected by salt concentration and the degradation effects were better when the mass fraction of NaCl in the culture medium was less than 3%. Further study showed that the strain PW could was resistant to high concentrations of some heavy metal ions.
KeywordIdentification Isolation of pyrene degrading strain PAHs
URLView the original
Language英語
Fulltext Access
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Beijing University of Chemical Technology
2.Universidade de Macau
3.Research Center for Eco-Environmental Sciences Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Xie W.,Lin A.,Yang X.,et al. Isolation, identification and characteristics of pyrene degrading bacteria Ochrobactrum sp.[J]. Beijing Huagong Daxue Xuebao (Ziran Kexueban)/Journal of Beijing University of Chemical Technology (Natural Science Edition),2011,38(3):76-80.
APA Xie W.,Lin A.,Yang X.,Wang F.,&Shim H..(2011).Isolation, identification and characteristics of pyrene degrading bacteria Ochrobactrum sp..Beijing Huagong Daxue Xuebao (Ziran Kexueban)/Journal of Beijing University of Chemical Technology (Natural Science Edition),38(3),76-80.
MLA Xie W.,et al."Isolation, identification and characteristics of pyrene degrading bacteria Ochrobactrum sp.".Beijing Huagong Daxue Xuebao (Ziran Kexueban)/Journal of Beijing University of Chemical Technology (Natural Science Edition) 38.3(2011):76-80.
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