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Removal of carbamazepine and naproxen by immobilized Phanerochaete chrysosporium under non-sterile condition
Li XQ1; de Toledo RA1; Wang SP2; Shim H1
Source PublicationNew Biotechnology
Other Abstract

This study explored the utilization of a white-rot fungus (WRF), Phanerochaete chrysosporium, immobilized in wood chips, to remove carbamazepine and naproxen under non-sterile condition. The removal efficiencies for both pharmaceutically active compounds (PhACs) in artificially contaminated water were improved by 4% for naproxen and 30% for carbamazepine in seven days, compared to without wood chips. Although adsorption was crucial at the early stage, bioremoval was found to be the main removal mechanism for both PhACs. The extracellular enzymes played important roles in the naproxen removal, while the intracellular enzyme system was responsible for the carbamazepine removal. The increased of intracellular enzyme activity through the immobilization of WRF cells may contribute to the significantly enhanced removal efficiency for carbamazepine. In addition, the removal of naproxen or carbamazepine slightly increased when both compounds coexisted, compared to the system where the two pharmaceuticals existed separately. Based on the batch experimental results, a fixed-bed bioreactor packed with a mixture of WRF mycelia pellets and wood chips was developed and operated with the intermittent feeding and continuous aerating mode for 28 days under non-sterile condition, with naproxen and carbamazepine spiked into the influent at 1.0mgL-1. Almost complete removal of naproxen and 60-80% removal of carbamazepine were obtained in the first two weeks. However, the removal efficiencies for both compounds suddenly dropped to as low as less than 20% by the 14th day, possibly due to the contamination by other microorganisms in the reactor. After the addition of 8.25% sodium hypochlorite at the ratio of 1:100 (v/v) into the influent tank on both Day 20 and Day 25, a rapid recovery (higher than 95%) was achieved in the naproxen removal, by effectively inhibiting contamination in the reactor. In comparison, the same rebounding phenomenon was not observed for carbamazepine and this difference may be associated to the various enzyme-working systems. A longer hydraulic retention time (HRT) was conducive to improve the removal of both compounds.

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Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemical Research Methods ; Biotechnology & Applied Microbiology
WOS IDWOS:000350896400006
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Cited Times [WOS]:21   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorShim H
Affiliation1.Univ Macau, Fac Sci & Technol, Dept Civil & Environm Engn, Macau, Peoples R China
2.Univ Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Macau, Peoples R China
First Author AffilicationUniversity of Macau
Corresponding Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Li XQ,de Toledo RA,Wang SP,et al. Removal of carbamazepine and naproxen by immobilized Phanerochaete chrysosporium under non-sterile condition[J]. New Biotechnology,2015,32(2):282-289.
APA Li XQ,de Toledo RA,Wang SP,&Shim H.(2015).Removal of carbamazepine and naproxen by immobilized Phanerochaete chrysosporium under non-sterile condition.New Biotechnology,32(2),282-289.
MLA Li XQ,et al."Removal of carbamazepine and naproxen by immobilized Phanerochaete chrysosporium under non-sterile condition".New Biotechnology 32.2(2015):282-289.
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