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Garcinone E induces apoptosis and inhibits migration and invasion in ovarian cancer cells
Xu, Xiao-Huang1; Liu, Qian-Yu1,2; Li, Ting1; Liu, Jian-Lin1; Chen, Xin1; Huang, Li1; Qiang, Wen-An3,4; Chen, Xiuping1; Wang, Yitao1; Lin, Li-Gen1; Lu, Jin-Jian1
2017-09-06
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume7
Abstract

Ovarian cancer remains the most lethal gynecological malignant tumor. In this study, 24 xanthones were isolated and identified from the pericarps of mangosteen (Garcinia mangostana), and their anti-proliferative activities were tested in ovarian cancer cells. Garcinone E (GE) was found to exhibit excellent anti-proliferative effects among the tested xanthones. It significantly inhibited the proliferation in HEY, A2780, and A2780/Taxol cells as evidenced by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, lactate dehydrogenase (LDH) release assay, Hoechst 33342 staining, annexin V/PI staining, and JC-1 staining. It induced endoplasmic reticulum (ER) stress and activated the protective inositol-requiring kinase (IRE)-1 alpha pathway. Knocking down IRE-1 alpha further activated the caspase cascade and caused an increase in cell death. Moreover, GE eliminated the migratory ability of HEY cells by reducing the expression of RhoA and Rac. It also blocked the invasion, which might be related to downregulation of matrix metalloproteinases (MMPs), i.e., MMP-9 and MMP-2, and upregulation of tissue inhibitors of metalloproteinase (TIMP) -1 and TIMP-2. In summary, GE exerts anticancer activities by inducing apoptosis and suppressing migration and invasion in ovarian cancer cells, which indicates its therapeutic potential for ovarian cancer.

DOIhttp://doi.org/10.1038/s41598-017-11417-4
URLView the original
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000409439900136
PublisherNATURE PUBLISHING GROUP
The Source to ArticleWOS
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Cited Times [WOS]:6   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLin, Li-Gen; Lu, Jin-Jian
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
2.Guangdong Medical Device Quality Surveillance and Test Institute, Guangzhou, Guangdong, China
3.Division of Reproductive Science in Medicine, Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA
4.Center for Developmental Therapeutics, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, USA
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Xu, Xiao-Huang,Liu, Qian-Yu,Li, Ting,et al. Garcinone E induces apoptosis and inhibits migration and invasion in ovarian cancer cells[J]. SCIENTIFIC REPORTS,2017,7.
APA Xu, Xiao-Huang.,Liu, Qian-Yu.,Li, Ting.,Liu, Jian-Lin.,Chen, Xin.,...&Lu, Jin-Jian.(2017).Garcinone E induces apoptosis and inhibits migration and invasion in ovarian cancer cells.SCIENTIFIC REPORTS,7.
MLA Xu, Xiao-Huang,et al."Garcinone E induces apoptosis and inhibits migration and invasion in ovarian cancer cells".SCIENTIFIC REPORTS 7(2017).
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