UM
Recognition-then-Reaction Enables Site-Selective Bioconjugation to Proteins on Live-Cell Surfaces
Cui, Cheng; Zhang, Hui; Wang, Ruowen; Cansiz, Sena; Pan, Xiaoshu; Wan, Shuo; Hou, Weijia; Li, Long; Chen, Meiwan; Liu, Yuan; Chen, Xigao; Liu, Qiaoling; Tan, Weihong
2017-09-18
Source PublicationANGEWANDTE CHEMIE-INTERNATIONAL EDITION
ISSN1433-7851
Volume56Issue:39Pages:11954-11957
AbstractSite-selective protein modification is a key step in facilitating protein functionalization and manipulation. To accomplish this, genetically engineered proteins were previously required, but the procedure was laborious, complex, and technically challenging. Herein we report the development of aptamer-based recognition-then-reaction to guide site-selective protein/DNA conjugation in a single step with outstanding selectivity and efficiency. As models, several proteins, including human thrombin, PDGF-BB, Avidin, and His-tagged recombinant protein, were studied, and the results showed excellent selectivity under mild reaction conditions. Taking advantage of aptamers as recognition elements with extraordinary selectivity and affinity, this simple preparation method can tag a protein in a complex milieu. Thus, with the aptamer obtained from cell-SELEX, real-time modification of live-cell membrane proteins can be achieved in one step without any pre-treatment.
Keywordaptamer template cell-surface modification protein conjugation site-selective
DOI10.1002/anie.201706285
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000410810600052
PublisherWILEY-V C H VERLAG GMBH
The Source to ArticleWOS
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Cui, Cheng,Zhang, Hui,Wang, Ruowen,et al. Recognition-then-Reaction Enables Site-Selective Bioconjugation to Proteins on Live-Cell Surfaces[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2017,56(39):11954-11957.
APA Cui, Cheng.,Zhang, Hui.,Wang, Ruowen.,Cansiz, Sena.,Pan, Xiaoshu.,...&Tan, Weihong.(2017).Recognition-then-Reaction Enables Site-Selective Bioconjugation to Proteins on Live-Cell Surfaces.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,56(39),11954-11957.
MLA Cui, Cheng,et al."Recognition-then-Reaction Enables Site-Selective Bioconjugation to Proteins on Live-Cell Surfaces".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 56.39(2017):11954-11957.
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