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Isolation, identification, and characterization of the nematophagous fungus Monacrosporium salinum from China
Liu W.; Han Y.; Wang B.-B.; Sun L.-J.; Chen M.-Y.; Cai K.-Z.; Li X.; Zhao M.-W.; Xu C.-L.; Xu Q.; Yi L.-X.; Wang H.; Xie D.-Q.; Li X.-S.; Wu J.-Y.; Yang J.; Wei S.; Li D.; Chen C.-R.; Zheng T.-H.; Li Q.; Peng J.-W.
2015
Source PublicationJournal of Basic Microbiology
ISSN15214028 0233111X
Volume55Issue:8Pages:992-1001
AbstractNematophagous fungi are considered to have the best potential as biological agents for the control of gastrointestinal nematodes in domestic animals. However, relatively few studies have been conducted with the genus Monacrosporium, especially with strains native to China. In the present study, we isolated and identified nematophagous fungi from fresh sheep feces. A pure fungal strain was molecularly characterized, and its nematophagous activity was evaluated. The morphological plasticity of the isolated strain, as well as its interaction with the nematode targets, was observed by scanning electron microscopy of the infected Trichostrongylus colubriformis L3 and the free-living nematode Caenorhabditis elegans. Three isolated fungal strains from the 30 fresh fecal samples of sheep from Inner Mongolia, China exhibited predatory activity; however, only a single strain was successfully purified (SF 0459). The SF 0459 strain was characterized by morphological analysis of its conidia and sequencing of its ITS1-5.8S rDNA-ITS2 region. This strain was identified to be Monacrosporium salinum (GenBank ID: KP036623). Nematophagous fungus helper bacteria were found at the interaction points between fungi and nematodes. The percentage of live T. colubriformis L3 was reduced by 83.79-88.69% based on the in vitro assay.
KeywordBiological control Caenorhabditis elegans Monacrosporium salinum Nematophagous fungus Trichostrongylus colubriformis
DOI10.1002/jobm.201400909
URLView the original
Language英語
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
AffiliationNorthwest University for Nationalities
Recommended Citation
GB/T 7714
Liu W.,Han Y.,Wang B.-B.,et al. Isolation, identification, and characterization of the nematophagous fungus Monacrosporium salinum from China[J]. Journal of Basic Microbiology,2015,55(8):992-1001.
APA Liu W..,Han Y..,Wang B.-B..,Sun L.-J..,Chen M.-Y..,...&Peng J.-W..(2015).Isolation, identification, and characterization of the nematophagous fungus Monacrosporium salinum from China.Journal of Basic Microbiology,55(8),992-1001.
MLA Liu W.,et al."Isolation, identification, and characterization of the nematophagous fungus Monacrosporium salinum from China".Journal of Basic Microbiology 55.8(2015):992-1001.
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