UM  > 中華醫藥研究院
Toosendanin, a natural product, inhibited TGF-1-induced epithelial-mesenchymal transition through ERK/Snail pathway
Luo, Weiwei; Liu, Xin; Sun, Wen; Lu, Jin-Jian; Wang, Yitao; Chen, Xiuping
2018-10
Source PublicationPHYTOTHERAPY RESEARCH
ISSN0951-418X
Volume32Issue:10Pages:2009-2020
Abstract

Epithelial-mesenchymal transition (EMT) plays important roles in the metastasis of solid tumors. In this study, the effect of toosendanin (TSN), a natural insecticide extracted from Melia toosendan Sieb et Zucc, on transforming growth factor-1 (TGF-1)-induced EMT was investigated. EMT was induced by TGF-1 in A549 and H1975 lung cancer cells. The morphological alterations were observed with a microscopy. The protein expression and localization of EMT biomarkers were determined by Western blotting and immunofluorescence. The migration, invasion, and adhesion were determined by wound-healing, transwell, and adhesion assays. TGF-1 treatment induced spindle-shaped alterations of cells, upregulation of N-cadherin, Vimentin, p-ERK1/2, and downregulation of E-cadherin. The abilities of migration, invasion, and adhesion were also enhanced. These effects were significantly reversed by TSN at very low concentration (<10nM). Furthermore, silence Snail significantly reversed TGF-1-induced EMT biomarkers. In addition, TGF-1-induced phosphorylation of ERK1/2 without affecting p38 mitogen-activated protein kinases and Jun N-terminal kinase. PD98059 and U0126, inhibitors of ERK1/2, showed similar inhibitory effect to that of TSN. In summary, TSN significantly inhibited TGF-1-induced EMT and migration, invasion, and adhesion through ERK/Snail pathway in lung cancer cells. This study provides novel anticancer effects and molecular mechanisms for TSN.

KeywordEmt Erk1/2 Snail Lung Cancer Toosendanin
DOIhttp://doi.org/10.1002/ptr.6132
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal ; Pharmacology & Pharmacy
WOS IDWOS:000446565300014
PublisherWILEY
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorWang, Yitao; Chen, Xiuping
AffiliationState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Luo, Weiwei,Liu, Xin,Sun, Wen,et al. Toosendanin, a natural product, inhibited TGF-1-induced epithelial-mesenchymal transition through ERK/Snail pathway[J]. PHYTOTHERAPY RESEARCH,2018,32(10):2009-2020.
APA Luo, Weiwei,Liu, Xin,Sun, Wen,Lu, Jin-Jian,Wang, Yitao,&Chen, Xiuping.(2018).Toosendanin, a natural product, inhibited TGF-1-induced epithelial-mesenchymal transition through ERK/Snail pathway.PHYTOTHERAPY RESEARCH,32(10),2009-2020.
MLA Luo, Weiwei,et al."Toosendanin, a natural product, inhibited TGF-1-induced epithelial-mesenchymal transition through ERK/Snail pathway".PHYTOTHERAPY RESEARCH 32.10(2018):2009-2020.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Luo, Weiwei]'s Articles
[Liu, Xin]'s Articles
[Sun, Wen]'s Articles
Baidu academic
Similar articles in Baidu academic
[Luo, Weiwei]'s Articles
[Liu, Xin]'s Articles
[Sun, Wen]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Luo, Weiwei]'s Articles
[Liu, Xin]'s Articles
[Sun, Wen]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 10.1002_ptr.6132.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.