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Toosendanin, a natural product, inhibited TGF-1-induced epithelial-mesenchymal transition through ERK/Snail pathway
Luo, Weiwei; Liu, Xin; Sun, Wen; Lu, Jin-Jian; Wang, Yitao; Chen, Xiuping
2018-10
Source PublicationPHYTOTHERAPY RESEARCH
ISSN0951-418X
Volume32Issue:10Pages:2009-2020
AbstractEpithelial-mesenchymal transition (EMT) plays important roles in the metastasis of solid tumors. In this study, the effect of toosendanin (TSN), a natural insecticide extracted from Melia toosendan Sieb et Zucc, on transforming growth factor-1 (TGF-1)-induced EMT was investigated. EMT was induced by TGF-1 in A549 and H1975 lung cancer cells. The morphological alterations were observed with a microscopy. The protein expression and localization of EMT biomarkers were determined by Western blotting and immunofluorescence. The migration, invasion, and adhesion were determined by wound-healing, transwell, and adhesion assays. TGF-1 treatment induced spindle-shaped alterations of cells, upregulation of N-cadherin, Vimentin, p-ERK1/2, and downregulation of E-cadherin. The abilities of migration, invasion, and adhesion were also enhanced. These effects were significantly reversed by TSN at very low concentration (<10nM). Furthermore, silence Snail significantly reversed TGF-1-induced EMT biomarkers. In addition, TGF-1-induced phosphorylation of ERK1/2 without affecting p38 mitogen-activated protein kinases and Jun N-terminal kinase. PD98059 and U0126, inhibitors of ERK1/2, showed similar inhibitory effect to that of TSN. In summary, TSN significantly inhibited TGF-1-induced EMT and migration, invasion, and adhesion through ERK/Snail pathway in lung cancer cells. This study provides novel anticancer effects and molecular mechanisms for TSN.
KeywordEMT ERK1 2 lung cancer Snail toosendanin
DOI10.1002/ptr.6132
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal ; Pharmacology & Pharmacy
WOS IDWOS:000446565300014
PublisherWILEY
The Source to ArticleWOS
Citation statistics
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Luo, Weiwei,Liu, Xin,Sun, Wen,et al. Toosendanin, a natural product, inhibited TGF-1-induced epithelial-mesenchymal transition through ERK/Snail pathway[J]. PHYTOTHERAPY RESEARCH,2018,32(10):2009-2020.
APA Luo, Weiwei,Liu, Xin,Sun, Wen,Lu, Jin-Jian,Wang, Yitao,&Chen, Xiuping.(2018).Toosendanin, a natural product, inhibited TGF-1-induced epithelial-mesenchymal transition through ERK/Snail pathway.PHYTOTHERAPY RESEARCH,32(10),2009-2020.
MLA Luo, Weiwei,et al."Toosendanin, a natural product, inhibited TGF-1-induced epithelial-mesenchymal transition through ERK/Snail pathway".PHYTOTHERAPY RESEARCH 32.10(2018):2009-2020.
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