UM
Using an online phycocyanin fluorescence probe for rapid monitoring of cyanobacteria in Macau freshwater reservoir
Yijun Kong1; Inchio Lou2; Yiyong Zhang1; Chong U. Lou1; Kai Meng Mok2
2014-12
Conference Name1st international conference on Challenges in Aquatic Sciences
Source PublicationHydrobiologia
Volume741
Issue1
Pages33-49
Conference DateMAR 15-21, 2013
Conference PlaceKeelung, TAIWAN
PublisherSPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
Abstract

Monitoring of cyanobacteria and their toxins are traditionally conducted by cell counting, chlorophyll-a (chl-a) determination and cyanotoxin measurements, respectively. These methods are tedious, costly, time consuming, and insensitive to rapid changes in water quality and cyanobacterial abundance. We have applied and tested an online phycocyanin (PC) fluorescence probe for rapid monitoring of cyanobacteria in the Macau Storage Reservoir (MSR) that is experiencing cyanobacterial blooms. The relationships among cyanobacterial abundance, biovolume, cylindrospermopsin concentration, and PC fluorescence were analyzed using both laboratory and in-the-field studies. The performance of the probe was compared with traditional methods, and its advantages and limitations were assessed in pure and mixed cyanobacterial cultures in the laboratory. The proposed techniques successfully estimated the species including Microcystis and Cylindrospermopsis, two toxic species recently observed in the MSR. During February–November, 2010, the PC probe detected high correlations between PC and cell numbers (R = 0.71). Unlike the chl-a content, which indicates only the total algal biomass, the PC pigment specifically indicates cyanobacteria. These results support the PC parameter as a reliable estimate of cyanobacterial cell number, especially in freshwater bodies where the phytoplankton community and structure are stable. Thus, the PC probe is potentially applicable to online monitoring of cyanobacteria.

KeywordCyanobacteria Freshwater Reservoir Online Measurement Phycocyanin Fluorescence Probe
DOIhttps://doi.org/10.1007/s10750-013-1759-3
URLView the original
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology
WOS SubjectMarine & Freshwater Biology
WOS IDWOS:000343998000005
Fulltext Access
Citation statistics
Cited Times [WOS]:18   [WOS Record]     [Related Records in WOS]
Document TypeConference paper
CollectionUniversity of Macau
Affiliation1.Laboratory & Research Center, Macao Water Co. Ltd., 718 Avenida do ConselheiroBorja, Macau SAR, China
2.Department of Civil and Environmental Engineering, Faculty of Science and Technology, University of Macau, Av. Padre Toma´s Pereira Taipa, Macau SAR, China
Recommended Citation
GB/T 7714
Yijun Kong,Inchio Lou,Yiyong Zhang,et al. Using an online phycocyanin fluorescence probe for rapid monitoring of cyanobacteria in Macau freshwater reservoir[C]:SPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS,2014:33-49.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yijun Kong]'s Articles
[Inchio Lou]'s Articles
[Yiyong Zhang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yijun Kong]'s Articles
[Inchio Lou]'s Articles
[Yiyong Zhang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yijun Kong]'s Articles
[Inchio Lou]'s Articles
[Yiyong Zhang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.