UM
Genome-guided and mass spectrometry investigation of natural products produced by a potential new actinobacterial strain isolated from a mangrove ecosystem in Futian, Shenzhen, China
Dini Hu1; Cheng Gao2; Chenghang Sun3; Tao Jin4; Guangyi Fan4; Kai Meng Mok1; Simon MingYuen Lee2,5
2019-01-29
Source PublicationScientific Reports
ISSN2045-2322
Volume9Issue:1
Other Abstract

Actinobacteria, a group of gram-positive bacteria, can produce plenty of valuable bioactive secondary metabolites, especially antibiotics. Hence, in order to search for new actinobacteria, actinobacterial isolates were obtained from rhizosphere soil collected from the Futian mangrove ecosystem in Shenzhen, China. According to 16S rRNA sequences, 14 actinobacterial strains of the genus Streptomyces, Rhodococcus, Microbacterium, Micromonospora, Actinoplanes and Mycobacterium were isolated and identified. Among these, strain Mycobacterium sp.13 was described as a potential new species belonging to the genus Mycobacterium within the class of actinobacteria according to the genomic analysis. The genome-based 16S rRNA sequences had 98.48% sequence similarity with Mycobacterium moriokaense DSM 44221. Meanwhile, the genome sequences of Mycobacterium sp.13 showed an average nucleotide identity (ANI) with the Mycobacterium mageritense DSM 44476, Mycobacterium smegmatis MKD8 and Mycobacterium goodii strain X7B of only 74.79%, 76.12% and 76.42%, respectively. Furthermore, genome-mining results showed that Mycobacterium sp.13 contained 105 gene clusters encoding to the secondary metabolite biosynthesis, where many kinds of terpene, bacteriocin, T1pks, Nrps, saccharide, fatty acid, butyrolactone, ectoine and resorcinol were included. Finally, through LC-MS and HR-MS, analyzing the small molecules from ethyl acetate extract of this strain, asukamycin C and apramycin were for the first time found present to be in Mycobacterium moriokaense strain. Our study provides evidence in support of the potential new Mycobacterium sp.13 isolated from the mangrove environment as a possible novel source of natural products.

DOIhttps://doi.org/10.1038/s41598-018-37475-w
URLView the original
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000456955500003
PublisherNATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorSimon MingYuen Lee
Affiliation1.Faculty of Science and Technology, Department of Civil and Environmental Engineering, University of Macau, Macao, China.
2.State Key Laboratory of Quality Research in Chinese Medicine and institute of Chinese Medical Sciences, University of Macau, Macao, China.
3.Institute of Medicinal Biotechnology, Chinese Academy of Medical Science & Peking Union Medical College, Tiantanxili No 1, Beijing, 100050, P. R. China.
4.Beijing Genome Institute– Shenzhen, Shenzhen, 518083, China.
5.Present address: Institute of Chinese Medical Sciences, University of Macau, Avenida da Universidade, Room No N22-7003. N22 Building, Taipa, Macao, China.
First Author AffilicationFaculty of Science and Technology
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Dini Hu,Cheng Gao,Chenghang Sun,et al. Genome-guided and mass spectrometry investigation of natural products produced by a potential new actinobacterial strain isolated from a mangrove ecosystem in Futian, Shenzhen, China[J]. Scientific Reports,2019,9(1).
APA Dini Hu.,Cheng Gao.,Chenghang Sun.,Tao Jin.,Guangyi Fan.,...&Simon MingYuen Lee.(2019).Genome-guided and mass spectrometry investigation of natural products produced by a potential new actinobacterial strain isolated from a mangrove ecosystem in Futian, Shenzhen, China.Scientific Reports,9(1).
MLA Dini Hu,et al."Genome-guided and mass spectrometry investigation of natural products produced by a potential new actinobacterial strain isolated from a mangrove ecosystem in Futian, Shenzhen, China".Scientific Reports 9.1(2019).
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