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Evaluation in zebrafish model of the toxicity of rhodamine B-conjugated crotamine, a peptide potentially useful for diagnostics and therapeutics
Chan, Judy Yuet-Wa; Zhou, Hefeng; Kwan, Yiu Wa; Chan, Shun Wan; Radis-Baptista, Gandhi; Lee, Simon Ming-Yuen
2017-11
Source PublicationJOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
ISSN1095-6670
Volume31Issue:11
AbstractCrotamine is defensin-like cationic peptide from rattlesnake venom that possesses anticancer, antimicrobial, and antifungal properties. Despite these promising biological activities, toxicity is a major concern associated with the development of venom-derived peptides as therapeutic agents. In the present study, we used zebrafish as a system model to evaluate the toxicity of rhodamine B-conjugated (RhoB) crotamine derivative. The lethal toxic concentration of RhoB-crotamine was as low as 4M, which effectively kill zebrafish larvae in less than 10min. With non-lethal concentrations (<1M), crotamine caused malformation in zebrafish embryos, delayed or completely halted hatching, adversely affected embryonic developmental programming, decreased the cardiac functions, and attenuated the swimming distance of zebrafish. The RhoB-crotamine translocated across vitelline membrane and accumulated in zebrafish yolk sac. These results demonstrate the sensitive responsivity of zebrafish to trial crotamine analogues for the development of novel therapeutic peptides with improved safety, bioavailability, and efficacy profiles.
Keywordacute toxicity rhodamine B-crotamine screening of pharmaceuticals venom-derived peptides zebrafish model
DOI10.1002/jbt.21964
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Toxicology
WOS SubjectBiochemistry & Molecular Biology ; Toxicology
WOS IDWOS:000414685200007
PublisherWILEY
The Source to ArticleWOS
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
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GB/T 7714
Chan, Judy Yuet-Wa,Zhou, Hefeng,Kwan, Yiu Wa,et al. Evaluation in zebrafish model of the toxicity of rhodamine B-conjugated crotamine, a peptide potentially useful for diagnostics and therapeutics[J]. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,2017,31(11).
APA Chan, Judy Yuet-Wa,Zhou, Hefeng,Kwan, Yiu Wa,Chan, Shun Wan,Radis-Baptista, Gandhi,&Lee, Simon Ming-Yuen.(2017).Evaluation in zebrafish model of the toxicity of rhodamine B-conjugated crotamine, a peptide potentially useful for diagnostics and therapeutics.JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,31(11).
MLA Chan, Judy Yuet-Wa,et al."Evaluation in zebrafish model of the toxicity of rhodamine B-conjugated crotamine, a peptide potentially useful for diagnostics and therapeutics".JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY 31.11(2017).
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