Molecular characterization of branched polysaccharides from Tremella fuciformis by asymmetrical flow field-flow fractionation and size exclusion chromatography

doi:10.1002/jssc.201700615

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	Molecular characterization of branched polysaccharides from Tremella fuciformis by asymmetrical flow field-flow fractionation and size exclusion chromatography
	Wu, Ding-Tao; Deng, Yong; Zhao, Jing; Li, Shao-Ping
	2017-11
Source Publication	JOURNAL OF SEPARATION SCIENCE
ISSN	1615-9306
Volume	40 Issue:21 Pages:4272-4280
Abstract	To accurately characterize branched polysaccharides with high molecular weights from medicinal and edible mushrooms and identify the limitations of size exclusion chromatography, molecular characteristics of polysaccharides from Tremella fuciformis were determined and compared by asymmetrical flow field-flow fractionation coupled with multiangle laser light scattering and refractive index detection, and size exclusion chromatography coupled with multiangle laser light scattering and refractive index detection, respectively. Results showed that molecular weights of three batches of T. fuciformis polysaccharides were determined as 2.167 x 10(6) (TF1), 2.334 x 10(6) (TF2), and 2.435 x 10(6) Da (TF3) by size exclusion chromatography, and 3.432 x 10(6) (TF1), 3.739 x 10(6) (TF2), and 3.742 x 10(6) Da (TF3) by asymmetrical flow field-flow fractionation, as well as 3.469 x 10(6) Da (TF1) by off-line multiangle laser light scattering, respectively. Results suggested that size exclusion chromatography was unable to accurately characterize T. fuciformis polysaccharides, which may be due to its limitations such as shear degradation and abnormal coelution. Compared to size exclusion chromatography, asymmetrical flow field-flow fractionation could be a better technique for the molecular characterization of branched polysaccharides with high molecular weights from medicinal and edible mushrooms, as well as from other natural resources.
Keyword	asymmetrical flowfield-flow fractionation multiangle laser light scattering polysaccharides size exclusion chromatography Tremella fuciformis
DOI	10.1002/jssc.201700615
URL	View the original
Indexed By	SCI
Language	英语
WOS Research Area	Chemistry
WOS Subject	Chemistry, Analytical
WOS ID	WOS:000414701200019
Publisher	WILEY-V C H VERLAG GMBH
The Source to Article	WOS
Fulltext Access	View Full-Text via DOI View Full-Text via Web of Science
Citation statistics	Cited Times [WOS]:4 [WOS Record] [Related Records in WOS]
Document Type	Journal article
Collection	Institute of Chinese Medical Sciences
Recommended Citation GB/T 7714	Wu, Ding-Tao,Deng, Yong,Zhao, Jing,et al. Molecular characterization of branched polysaccharides from Tremella fuciformis by asymmetrical flow field-flow fractionation and size exclusion chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(21):4272-4280.
APA	Wu, Ding-Tao,Deng, Yong,Zhao, Jing,&Li, Shao-Ping.(2017).Molecular characterization of branched polysaccharides from Tremella fuciformis by asymmetrical flow field-flow fractionation and size exclusion chromatography.JOURNAL OF SEPARATION SCIENCE,40(21),4272-4280.
MLA	Wu, Ding-Tao,et al."Molecular characterization of branched polysaccharides from Tremella fuciformis by asymmetrical flow field-flow fractionation and size exclusion chromatography".JOURNAL OF SEPARATION SCIENCE 40.21(2017):4272-4280.

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