UM  > 中華醫藥研究院
Molecular characterization of branched polysaccharides from Tremella fuciformis by asymmetrical flow field-flow fractionation and size exclusion chromatography
Wu, Ding-Tao; Deng, Yong; Zhao, Jing; Li, Shao-Ping
2017-11
Source PublicationJOURNAL OF SEPARATION SCIENCE
ISSN1615-9306
Volume40Issue:21Pages:4272-4280
AbstractTo accurately characterize branched polysaccharides with high molecular weights from medicinal and edible mushrooms and identify the limitations of size exclusion chromatography, molecular characteristics of polysaccharides from Tremella fuciformis were determined and compared by asymmetrical flow field-flow fractionation coupled with multiangle laser light scattering and refractive index detection, and size exclusion chromatography coupled with multiangle laser light scattering and refractive index detection, respectively. Results showed that molecular weights of three batches of T. fuciformis polysaccharides were determined as 2.167 x 10(6) (TF1), 2.334 x 10(6) (TF2), and 2.435 x 10(6) Da (TF3) by size exclusion chromatography, and 3.432 x 10(6) (TF1), 3.739 x 10(6) (TF2), and 3.742 x 10(6) Da (TF3) by asymmetrical flow field-flow fractionation, as well as 3.469 x 10(6) Da (TF1) by off-line multiangle laser light scattering, respectively. Results suggested that size exclusion chromatography was unable to accurately characterize T. fuciformis polysaccharides, which may be due to its limitations such as shear degradation and abnormal coelution. Compared to size exclusion chromatography, asymmetrical flow field-flow fractionation could be a better technique for the molecular characterization of branched polysaccharides with high molecular weights from medicinal and edible mushrooms, as well as from other natural resources.
Keywordasymmetrical flowfield-flow fractionation multiangle laser light scattering polysaccharides size exclusion chromatography Tremella fuciformis
DOI10.1002/jssc.201700615
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000414701200019
PublisherWILEY-V C H VERLAG GMBH
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wu, Ding-Tao,Deng, Yong,Zhao, Jing,et al. Molecular characterization of branched polysaccharides from Tremella fuciformis by asymmetrical flow field-flow fractionation and size exclusion chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(21):4272-4280.
APA Wu, Ding-Tao,Deng, Yong,Zhao, Jing,&Li, Shao-Ping.(2017).Molecular characterization of branched polysaccharides from Tremella fuciformis by asymmetrical flow field-flow fractionation and size exclusion chromatography.JOURNAL OF SEPARATION SCIENCE,40(21),4272-4280.
MLA Wu, Ding-Tao,et al."Molecular characterization of branched polysaccharides from Tremella fuciformis by asymmetrical flow field-flow fractionation and size exclusion chromatography".JOURNAL OF SEPARATION SCIENCE 40.21(2017):4272-4280.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wu, Ding-Tao]'s Articles
[Deng, Yong]'s Articles
[Zhao, Jing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wu, Ding-Tao]'s Articles
[Deng, Yong]'s Articles
[Zhao, Jing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wu, Ding-Tao]'s Articles
[Deng, Yong]'s Articles
[Zhao, Jing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.