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Pro-angiogenic effects of Ilexsaponin A1 on human umbilical vein endothelial cells in vitro and zebrafish in vivo
Li, Jingjing1; Zhang, Jinming2; Zou, Liang3; Lee, Simon Ming-Yuen4; Yang, Cui5; Seto, Sai-Wang6; Leung, George Pak-Heng1
2017-12
Source PublicationPHYTOMEDICINE
ISSN0944-7113
Volume36Pages:229-237
Abstract

Background

Ilexsaponin A1 is the major bioactive ingredient of Ilex pubescens Hook. et Arn. This plant has been conventionally used in Traditional Chinese Medicine for the treatment of cardiovascular diseases including stroke, coronary arterial disease, and peripheral vascular diseases.

Purpose
To investigate the pro-angiogenic effect of Ilexsaponin A1 and its mechanism of action.


Study design
Human umbilical vein endothelial cells (HUVECs) and transgenic zebrafish Tg(fli1:EGFP) were employed as an in vitro and in vivo model respectively.


Methods
Pro-angiogenic effects of Ilexsaponin A1 were examined by assessing endothelial cell proliferation, migration, invasion and tube formation. The mechanism of pro-angiogenic effects was investigated by measuring the expression level of various signalling proteins. Furthermore, vascular endothelial growth factor (VEGF) tyrosine kinase inhibitor II (VRI)-induced vascular insufficient transgenic zebrafish model was used to confirm the results of the HUVECs results in vivo.


Results
Ilexsaponin A1 significantly promoted cell proliferation, migration, invasion and tube formation in HUVECs, and rescued blood vessel loss in VRI-induced vascular insufficient zebrafish. Ilexsaponin A1 upregulated p-Akt, p-mTOR, p-Src, p-FAK, p-MEK, and p-Erk1/2 in HUVECs.


Conclusion
This study showed that Ilexsaponin A1 exhibits pro-angiogenic activity in HUVECs and VRI-induced vascular insufficient zebrafish, probably by activating Akt/mTOR, MAPK/ERK and Src- and FAK-dependent signalling pathways. The findings suggest that Ilexsaponin A1 and probably I. pubescens, a major source of Ilexsaponin A1, could be developed as a potential therapeutic agent for preventing or treating cardiovascular diseases and/or other diseases related to vascular insufficiency.

KeywordIlexsaponin A1 Ilex Pubescens Pro-angiogenesis Zebrafish Endothelial Cells
DOI10.1016/j.phymed.2017.10.006
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000415693500028
PublisherELSEVIER GMBH, URBAN & FISCHER VERLAG
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLee, Simon Ming-Yuen; Leung, George Pak-Heng
Affiliation1.Department of Pharmacology and Pharmacy, The University of Hong Kong, Hong Kong, China
2.College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China
3.School of Medicine, Chengdu University, Chengdu, China
4.State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, China
5.Ethnic Drug Screening & Pharmacology Center, Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education, Yunnan Minzu University, Kunming 650500, China
6.National Institute of Complementary Medicine, University of Western Sydney, Campbelltown, NSW, Australia
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Li, Jingjing,Zhang, Jinming,Zou, Liang,et al. Pro-angiogenic effects of Ilexsaponin A1 on human umbilical vein endothelial cells in vitro and zebrafish in vivo[J]. PHYTOMEDICINE,2017,36:229-237.
APA Li, Jingjing.,Zhang, Jinming.,Zou, Liang.,Lee, Simon Ming-Yuen.,Yang, Cui.,...&Leung, George Pak-Heng.(2017).Pro-angiogenic effects of Ilexsaponin A1 on human umbilical vein endothelial cells in vitro and zebrafish in vivo.PHYTOMEDICINE,36,229-237.
MLA Li, Jingjing,et al."Pro-angiogenic effects of Ilexsaponin A1 on human umbilical vein endothelial cells in vitro and zebrafish in vivo".PHYTOMEDICINE 36(2017):229-237.
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