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NMR–DMF: A modular nuclear magnetic resonance–digital microfluidics system for biological assays
Ka-Meng Lei1; Pui-In Mak1; Man-Kay Law1; Rui P. Martins1,2
2014-10-27
Source PublicationAnalyst
ISSN0003-2654
Volume139Issue:23Pages:6204-6213
Abstract

We present a modular nuclear magnetic resonance–digital microfluidics (NMR–DMF) system as a portable diagnostic platform for miniaturized biological assays. With increasing number of combinations between designed probes and a specific target, NMR has become an accurate and rapid assay tool, which is capable of detecting particular kinds of proteins, DNAs, bacteria and cells with a customized probe quantitatively. Traditional sample operation (e.g., manipulation and mixing) relied heavily on human efforts. We herein propose a modular NMR–DMF system to allow the electronic automation of multi-step reaction-screening protocols. A figure-8 shaped coil is proposed to enlarge the usable inner space of a portable magnet by 4.16 times, generating a radio frequency (RF) excitation field in the planar direction. By electronically managing the electro-wetting-on-dielectric (EWOD) effects over an electrode array, preloaded droplets with the inclusion of biological constituents and targets can be programmed to mix and be guided to the detection site (3.5 × 3.5 mm) for high-sensitivity NMR screening (static B field: 0.46 T, RF field: 1.43 mT per ampere), with the result (voltage signal) displayed in real-time. To show the system's utility, automated real-time identification of 100 pM of avidin in a 14 μL droplet was achieved. The system shows promise as a robust and portable diagnostic device for a wide variety of biological analyses and screening applications. 

DOIhttp://doi.org/10.1039/c4an01285b
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000344523800021
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Citation statistics
Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionDEPARTMENT OF ELECTRICAL AND COMPUTER ENGINEERING
INSTITUTE OF MICROELECTRONICS
Corresponding AuthorPui-In Mak
Affiliation1.State-Key Laboratory of Analog and Mixed-Signal VLSI and FST-ECE, University of Macau, China
2.Instituto Superior Técnico, University of Lisbon, Portugal
First Author AffilicationUniversity of Macau
Corresponding Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Ka-Meng Lei,Pui-In Mak,Man-Kay Law,et al. NMR–DMF: A modular nuclear magnetic resonance–digital microfluidics system for biological assays[J]. Analyst,2014,139(23):6204-6213.
APA Ka-Meng Lei,Pui-In Mak,Man-Kay Law,&Rui P. Martins.(2014).NMR–DMF: A modular nuclear magnetic resonance–digital microfluidics system for biological assays.Analyst,139(23),6204-6213.
MLA Ka-Meng Lei,et al."NMR–DMF: A modular nuclear magnetic resonance–digital microfluidics system for biological assays".Analyst 139.23(2014):6204-6213.
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