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A palm-size μnMR relaxometer using a digital microfluidic device and a semiconductor transceiver for chemical/biological diagnosis
Ka-Meng Lei1,2; Pui-In Mak1,2; Man-Kay Law1; Rui P. Martins1,2,3
2015-02-05
Source PublicationAnalyst
ISSN13645528 00032654
Volume140Issue:15Pages:5129-5137
Abstract

Herein, we describe a micro-nuclear magnetic resonance (μNMR) relaxometer miniaturized to palm-size and electronically automated for multi-step and multi-sample chemical/biological diagnosis. The co-integration of microfluidic and microelectronic technologies enables an association between the droplet managements and μNMR assays inside a portable sub-Tesla magnet (1.2 kg, 0.46 Tesla). Targets in unprocessed biological samples, captured by specific probe-decorated magnetic nanoparticles (NPs), can be sequentially quantified by their spin-spin relaxation time (T) via multiplexed μNMR screening. Distinct droplet samples are operated by a digital microfluidic device that electronically manages the electrowetting-on-dielectric effects over an electrode array. Each electrode (3.5 × 3.5 mm) is scanned with capacitive sensing to locate the distinct droplet samples in real time. A cross-domain-optimized butterfly-coil-input semiconductor transceiver transduces between magnetic and electrical signals to/from a sub-10 μL droplet sample for high-sensitivity μNMR screening. A temperature logger senses the ambient temperature (0 to 40 °C) and a backend processor calibrates the working frequency for the transmitter to precisely excite the protons. In our experiments, the μNMR relaxometer quantifies avidin using biotinylated Iron NPs (Φ: 30 nm, [Fe]: 0.5 mM) with a sensitivity of 0.2 μM. Auto-handling and identification of two targets (avidin and water) are demonstrated and completed within 2.2 min. This μNMR relaxometer holds promise for combinatorial chemical/biological diagnostic protocols using closed-loop electronic automation.

DOIhttp://doi.org/10.1039/c5an00500k
URLView the original
Indexed BySCI
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000357810800026
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Citation statistics
Cited Times [WOS]:15   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionDEPARTMENT OF ELECTRICAL AND COMPUTER ENGINEERING
INSTITUTE OF MICROELECTRONICS
Corresponding AuthorPui-In Mak
Affiliation1.State-Key Laboratory of Analog and Mixed-Signal VLSI, University of Macau, China
2.Faculty of Science and Technology, Dept. of ECE, University of Macau, China
3.On leave from Instituto Superior Técnico, Universidade de Lisboa, Portugal
First Author AffilicationUniversity of Macau;  Faculty of Science and Technology
Corresponding Author AffilicationUniversity of Macau;  Faculty of Science and Technology
Recommended Citation
GB/T 7714
Ka-Meng Lei,Pui-In Mak,Man-Kay Law,et al. A palm-size μnMR relaxometer using a digital microfluidic device and a semiconductor transceiver for chemical/biological diagnosis[J]. Analyst,2015,140(15):5129-5137.
APA Ka-Meng Lei,Pui-In Mak,Man-Kay Law,&Rui P. Martins.(2015).A palm-size μnMR relaxometer using a digital microfluidic device and a semiconductor transceiver for chemical/biological diagnosis.Analyst,140(15),5129-5137.
MLA Ka-Meng Lei,et al."A palm-size μnMR relaxometer using a digital microfluidic device and a semiconductor transceiver for chemical/biological diagnosis".Analyst 140.15(2015):5129-5137.
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