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Sub-7-second genotyping of single-nucleotide polymorphism by high-resolution melting curve analysis on a thermal digital microfluidic device
Tianlan Chen1,2; Yanwei Jia1; Cheng Dong1,2; Jie Gao1,2; Pui-In Mak1,2; Rui P. Martins1,2,3
2016
Source PublicationLab on a Chip
ISSN1473-0197
Volume16Issue:4Pages:743-752
Abstract

We developed a thermal digital microfluidic (T-DMF) device enabling ultrafast DNA melting curve analysis (MCA). Within 7 seconds, the T-DMF device succeeded in differentiating a melting point difference down to 1.6 °C with a variation of 0.3°C in a tiny droplet sample (1.2 μL), which was 300 times faster and with 20 times less sample spending than the standard MCA (35 minutes, 25 μL) run in a commercial qPCR machine. Such a performance makes it possible for a rapid discrimination of single-nucleotide mutation relevant to prompt clinical decision-making. Also, aided by electronic intelligent control, the T-DMF device facilitates sample handling and pipelining in an automatic serial manner. An optimized oval-shaped thermal electrode is introduced to achieve high thermal uniformity. A device-sealing technique averts sample contamination and permits uninterrupted chemical/biological reactions. Simple fabrication using a single chromium layer fulfills both the thermal and typical transport electrode requirements. Capable of thermally modulating DNA samples with ultrafast MCA, this T-DMF device has the potential for a wide variety of life science analyses, especially for disease diagnosis and prognosis.

DOIhttp://doi.org/10.1039/c5lc01533b
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology
WOS IDWOS:000370333200012
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Cited Times [WOS]:12   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionINSTITUTE OF MICROELECTRONICS
Corresponding AuthorPui-In Mak
Affiliation1.State-Key Laboratory of Analog and Mixed-Signal VLSI, University of Macau, Macao, China
2.Faculty of Science and Technology – ECE, University of Macau, Macao, China
3.on leave from Instituto Superior Técnico, Universidade de Lisboa, Portugal
First Author AffilicationUniversity of Macau;  Faculty of Science and Technology
Corresponding Author AffilicationUniversity of Macau;  Faculty of Science and Technology
Recommended Citation
GB/T 7714
Tianlan Chen,Yanwei Jia,Cheng Dong,et al. Sub-7-second genotyping of single-nucleotide polymorphism by high-resolution melting curve analysis on a thermal digital microfluidic device[J]. Lab on a Chip,2016,16(4):743-752.
APA Tianlan Chen,Yanwei Jia,Cheng Dong,Jie Gao,Pui-In Mak,&Rui P. Martins.(2016).Sub-7-second genotyping of single-nucleotide polymorphism by high-resolution melting curve analysis on a thermal digital microfluidic device.Lab on a Chip,16(4),743-752.
MLA Tianlan Chen,et al."Sub-7-second genotyping of single-nucleotide polymorphism by high-resolution melting curve analysis on a thermal digital microfluidic device".Lab on a Chip 16.4(2016):743-752.
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