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Cucurbitacin B suppresses metastasis mediated by reactive oxygen species (ROS) via focal adhesion kinase (FAK) in breast cancer MDA-MB-231 cells
Luo Wei-Wei; Zhao Wen-Wen; Lu Jin-Jian; Wang Yi-Tao; Chen Xiu-Ping
2018-01
Source PublicationCHINESE JOURNAL OF NATURAL MEDICINES
ISSN2095-6975
Volume16Issue:1Pages:10-19
Abstract

Metastasis is responsible for the majority of cancer-related deaths and prevention of metastasis remains a big challenge for cancer therapy. Cucurbitacin B (Cuc B) is a natural triterpenoid with potent anticancer activities while its effect on metastasis remains unclear. In the present study, the inhibitory effect and mechanisms of Cuc B on metastasis were investigated in MDA-MB-231 breast cancer cells. The cells were treated with or without Cuc B, and the cytotoxicity was determined by MTT assay. The effect of Cuc B on metastasis was evaluated with wound healing, transwell, and adhesion assays. Furthermore, the adhesion of cancer cells to endothelial cells was determined. The protein expression was determined by Western blotting. Cuc B (< 100 nmol.L-1) showed no obvious cytotoxicity to MDA-MB-231 cells, but significantly inhibited migration, invasion, and adhesion to Matrigel, fibronectin, type I collagen, and endothelial cells. Cuc B dramatically inhibited the phosphorylation of focal adhesion kinase (FAK) and paxillin in dose-and time-dependent manners. Furthermore, Cuc B induced intracellular reactive oxygen species (ROS) generation, which could be reduced by N-acetyl-l-cysteine (NAC). In addition, NAC pretreatment could reverse Cuc B-induced suppression of migration and adhesion, expression of FAK, but showed no effect on paxillin expression. In summary, Cuc B suppressed ROS-dependent metastasis through FAK pathway in breast cancer MDA-MB-231 cells, demonstrating novel mechanisms for the anticancer effects of Cuc B.

KeywordCucurbitacin b Metastasis Ros Fak Breast Cancer
DOIhttps://doi.org/10.1016/S1875-5364(18)30025-6
URLView the original
Indexed BySCI
Language英语
WOS Research AreaIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS SubjectIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000425295900002
PublisherCHINESE JOURNAL NATURAL MEDICINES
The Source to ArticleWOS
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
AffiliationState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Science, University of Macau, Macao 999078, China.
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Luo Wei-Wei,Zhao Wen-Wen,Lu Jin-Jian,et al. Cucurbitacin B suppresses metastasis mediated by reactive oxygen species (ROS) via focal adhesion kinase (FAK) in breast cancer MDA-MB-231 cells[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2018,16(1):10-19.
APA Luo Wei-Wei,Zhao Wen-Wen,Lu Jin-Jian,Wang Yi-Tao,&Chen Xiu-Ping.(2018).Cucurbitacin B suppresses metastasis mediated by reactive oxygen species (ROS) via focal adhesion kinase (FAK) in breast cancer MDA-MB-231 cells.CHINESE JOURNAL OF NATURAL MEDICINES,16(1),10-19.
MLA Luo Wei-Wei,et al."Cucurbitacin B suppresses metastasis mediated by reactive oxygen species (ROS) via focal adhesion kinase (FAK) in breast cancer MDA-MB-231 cells".CHINESE JOURNAL OF NATURAL MEDICINES 16.1(2018):10-19.
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