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LampPort: a handheld digital microfluidic device for loop-mediated isothermal amplification (LAMP)
Wan L.1; Gao J.1; Chen T.1; Dong C.1; Li H.1; Wen Y.-Z.2; Lun Z.-R.2; Jia Y.1; Mak P.-I.1; Martins R.P.1
2019-03-01
Source PublicationBiomedical Microdevices
ISSN15728781 13872176
Volume21Issue:1
AbstractA major goal in the development of point-of-care (POC) devices is to build them as portable to provide a rapid and effective determination for disease pathogens. In nucleic acid testing, an optical detection system used to monitor the product of nucleic acid amplification has always been a bulky accessory. In this work, we developed a handheld, automatic and detection system-free thermal digital microfluidic (DMF) device for DNA detection by loop-mediated isothermal amplification (LAMP). Droplet manipulation and real-time temperature control systems were integrated into a handheld device. The control software could be installed into any tablet and communicate with the device via Bluetooth. In the experimentation, we loaded 2-μl samples with an electrowetting force into sandwich-structured DMF chips, thereby considerably reducing reagent consumptions. After an on-chip LAMP reaction, we added a highly concentrated SYBR Green I droplet and mixed it with a reaction droplet to enable product detection with the naked eye. This step prevented aerosol contamination by avoiding the exposure of the reaction droplet to the air. Using a blood parasite Trypanosoma brucei as a model system, this system showed similar results as a commercial thermal cycler and could detect 40 copies per reaction of the DNA target. This low-cost, compact device removed the bulky optical system for DNA detection, thus enabling it to be well suited for POC testing.
KeywordDigital microfluidic system Handheld device Loop-mediated isothermal amplification (LAMP) Naked-eye visualisation
DOI10.1007/s10544-018-0354-9
URLView the original
Language英語
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Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Universidade de Macau
2.Sun Yat-Sen University
3.Instituto Superior Técnico
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GB/T 7714
Wan L.,Gao J.,Chen T.,et al. LampPort: a handheld digital microfluidic device for loop-mediated isothermal amplification (LAMP)[J]. Biomedical Microdevices,2019,21(1).
APA Wan L..,Gao J..,Chen T..,Dong C..,Li H..,...&Martins R.P..(2019).LampPort: a handheld digital microfluidic device for loop-mediated isothermal amplification (LAMP).Biomedical Microdevices,21(1).
MLA Wan L.,et al."LampPort: a handheld digital microfluidic device for loop-mediated isothermal amplification (LAMP)".Biomedical Microdevices 21.1(2019).
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