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Genome-wide transcriptional regulation and chromatin dynamics in response to nitrogen availability in Aspergillus nidulans
Damien J. Downes2; Zhengqiang Miao1; Djordje Djordjevic3; Vinita Deshpande3; Ang Li1; Kaeling Tan1; Grethel Y Busot2; Joshua W.K. Ho3; Richard B. Todd2; Koon Ho Wong1
2015
Conference Namethe 28th Fungal Genetics Conference
Source Publication28th Fungal Genetics Conference
Conference DateMarch 17-22, 2015
Conference PlacePacific Grove, CA
Abstract

Fungi adapt their metabolism to nitrogen nutrient availability primarily via global transcriptional control of nitrogen uptake and metabolic genes. In Aspergillus nidulans, the GATA transcription factor AreA activates genes for nitrogen metabolism in response to nitrogen limitation or nitrogen starvation. We have performed ChIP-seq of RNA polymerase II in wild type and an areAΔ mutant in the presence of ammonium and during nitrogen starvation to identify the genome-wide AreA-dependent gene expression program. Using ChIP-seq of HA-epitope-tagged AreA, we determined the pan-genomic direct targets of this global regulator. We have also mapped global chromatin dynamics and genome-wide chromatin modifications in response to nitrogen nutrient availability. Our data reveal nitrogen transport, metabolic and regulatory gene targets as well as new targets for AreA, including promoters of iron siderophore biosynthesis genes, heme metabolism genes, and secondary metabolism genes. Differential chromatin modifications occurred in response to nitrogen availability and were enriched in secondary metabolism genes.

Language英语
Fulltext Access
Document TypeConference paper
CollectionFaculty of Health Sciences
Affiliation1.Faculty of Health Sciences, University of Macau, Taipa, Macau
2.Department of Plant Pathology, Kansas State University, Manhattan KS, USA
3.Victor Chang Cardiac Research Institute, and the University of New South Wales, Sydney, NSW, Australia
Recommended Citation
GB/T 7714
Damien J. Downes,Zhengqiang Miao,Djordje Djordjevic,et al. Genome-wide transcriptional regulation and chromatin dynamics in response to nitrogen availability in Aspergillus nidulans[C],2015.
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