UM  > 健康科學學院
Molecular characterization of hepatitis B virus surface antigen mutants in Singapore patients with hepatocellular carcinoma and hepatitis B virus carriers negative for HBsAg but positive for anti-HBs
CHONG JIN OON1; WEI NING CHEN2; KEE TAI GOH5; STEVEN MESENAS3; HAN SEONG NG3; GILBERT CHIANG4; CLARENCE TAN4; SHIUAN KOH1; SIAW WEI TENG1; IVY TOH1; MEI CHUNG MOH1; KIAN SIM GOO1; KAELING TAN1; AI LIN LEONG AND1; GEK SAN TAN1
2002
Source PublicationJournal of Gastroenterology and Hepatology
ISSN0815-9319
Volume17Pages:S491-S496
Abstract

Background and Aims: Mutations on the a-determinant of hepatitis B virus surface antigen (HBsAg),capable of escaping detection and vaccination, are identified in HBsAg-positive/anti-HBs-positive vaccinated infants. We studied the prevalence of these mutants in HBsAg-negative/anti-HBc-positive chronic HBV carriers and patients with hepatocellular carcinoma (HCC).
Methods: DNA sequence coding for the antigenic a-determinant of HBsAg was amplified from either HCC genomic DNA or serum samples of the selected patients and sequenced. The replicative mutant genomes were reconstituted in vitro and their reactivity to commercial kits measured.
Results: Mutations within and/or outside the a-determinant were identified in patients seronegative for HBsAg. They were then reconstituted in vitro and transiently transfected into HepG2 cells. Culture medium containing secreted HBV viral particles was collected and assayed for their binding to commercial
kits. Drastic decrease of reactivity to these kits was seen with most of the identified mutations, including those located outside the a-determinant.
Conclusion: The existence of a more complex antigenic structure of HBsAg is indicated by the decreased reactivity to detection of mutations, some of which are outside the a-determinant, escape vaccination and may persist in seronegative patients. The high proportion of HBsAg mutants that are integrated in HCC genomes suggests a role of these mutants in hepatocarcinogenesis, possibly leading to mutant HBV-related HCC.

KeywordHepatitis b Hepatoma Seronegative Suface Mutants
DOIhttps://doi.org/10.1046/j.1440-1746.17.s4.16.x
Indexed BySCI
Language英语
WOS Research AreaGastroenterology & Hepatology
WOS SubjectGastroenterology & Hepatology
WOS IDWOS:000181081900013
全文获取链接
引用统计
被引频次[WOS]:20   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题Faculty of Health Sciences
Affiliation1.Ransome Research Laboratory
2.Department of Clinical Research
3.Department of Gastroenterology
4.Department of Pathology, Singapore General Hospital
5.Quarantine and Epidemiology Department, Ministry of the Environment, Singapore
推荐引用方式
GB/T 7714
CHONG JIN OON,WEI NING CHEN,KEE TAI GOH,et al. Molecular characterization of hepatitis B virus surface antigen mutants in Singapore patients with hepatocellular carcinoma and hepatitis B virus carriers negative for HBsAg but positive for anti-HBs[J]. Journal of Gastroenterology and Hepatology,2002,17:S491-S496.
APA CHONG JIN OON.,WEI NING CHEN.,KEE TAI GOH.,STEVEN MESENAS.,HAN SEONG NG.,...&GEK SAN TAN.(2002).Molecular characterization of hepatitis B virus surface antigen mutants in Singapore patients with hepatocellular carcinoma and hepatitis B virus carriers negative for HBsAg but positive for anti-HBs.Journal of Gastroenterology and Hepatology,17,S491-S496.
MLA CHONG JIN OON,et al."Molecular characterization of hepatitis B virus surface antigen mutants in Singapore patients with hepatocellular carcinoma and hepatitis B virus carriers negative for HBsAg but positive for anti-HBs".Journal of Gastroenterology and Hepatology 17(2002):S491-S496.
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
Google Scholar
中相似的文章 Google Scholar
[CHONG JIN OON]的文章
[WEI NING CHEN]的文章
[KEE TAI GOH]的文章
Baidu academic
中相似的文章 Baidu academic
[CHONG JIN OON]的文章
[WEI NING CHEN]的文章
[KEE TAI GOH]的文章
Bing Scholar
中相似的文章 Bing Scholar
[CHONG JIN OON]的文章
[WEI NING CHEN]的文章
[KEE TAI GOH]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。