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Molecular Cloning and mRNA Distribution of Pituitary Adenylate Cyclase‐activating Polypeptide (PACAP)/PACAP‐related Peptide in the Lungfish
Lee, LTO; Tam, JKV; Chan, DW; Chow, BKC
2009
Conference NameConference on Trends in Comparative Endocrinology and Neurobiology
Source PublicationTRENDS IN COMPARATIVE ENDOCRINOLOGY AND NEUROBIOLOGY
Volume1163
Pages209-214
Conference DateSEP 02-06, 2008
Conference PlaceGenoa, ITALY
Author of SourceVaudry, H; Roubos, EW; Coast, GM; Vallarino, M
Publication PlaceUSA
PublisherWILEY-BLACKWELL, COMMERCE PLACE, 350 MAIN STREET, MALDEN 02148, MA USA
Abstract

In this article, we report the isolation of a full-length cDNA clone encoding pituitary adenylatecyclase-activating polypeptide (PACAP)/PACAP-related peptide (PRP) from lungfish Protopterus dolloi. When comparing the deduced amino acid sequences, the lungfish PACAP was found to be highly conserved with other vertebrates; however, the PRP shares only lower levels of sequence identity with known PRP sequences. Consistently in phylogenetic analysis, the lungfish PRP, similar to sturgeon PRP, fails to cluster with other PRPs. In addition to the full-length clone, another cDNA encoding a short precursor that lacks the first 32 amino acids of the PRP was also isolated. Interestingly, similar isoforms were also identified in several nonmammalian vertebrates, and it was suggested that exon skipping of PRP/PACAP transcripts was a mechanism that regulated theexpression ratio of PACAP to PRP in nonmammalian vertebrates. By real-time PCR, both long and short PRP/PACAP transcripts were found almost exclusively in the brain, and the short isoform is themore abundant transcript (3.7 times more), indicating that PACAP is the major product produced inlungfish brain. The expression patterns of lungfish and previously studied frog PRP/PACAP suggest that the PRP/PACAP gene in the tetrapod lineage may first express in the central nervous system; inthe process of evolution, the functions of these peptides diversified and were later found in other tissues.

KeywordPituitary Adenylate Cyclase-activating Polypeptide Lungfish Pacap-related Peptide
DOIhttps://doi.org/10.1111/j.1749-6632.2008.03661.x
Indexed BySCI
Language英语
WOS Research AreaEndocrinology & Metabolism ; Science & Technology - Other Topics
WOS SubjectEndocrinology & Metabolism ; Multidisciplinary Sciences
WOS IDWOS:000266493400018
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeConference paper
CollectionFaculty of Health Sciences
AffiliationUniv Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Lee, LTO,Tam, JKV,Chan, DW,et al. Molecular Cloning and mRNA Distribution of Pituitary Adenylate Cyclase‐activating Polypeptide (PACAP)/PACAP‐related Peptide in the Lungfish[C]//Vaudry, H; Roubos, EW; Coast, GM; Vallarino, M. USA:WILEY-BLACKWELL, COMMERCE PLACE, 350 MAIN STREET, MALDEN 02148, MA USA,2009:209-214.
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