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Discovery of growth hormone-releasing hormones and receptors in nonmammalian vertebrates
Leo T. O. Lee1; Francis K. Y. Siu1; Janice K. V. Tam1; Ivy T. Y. Lau1; Anderson O. L. Wong1; Marie C. M. Lin2; Hubert Vaudry3; Billy K. C. Chow1
2007
Source PublicationPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN0027-8424
Volume104Issue:7Pages:2133-2138
Abstract

In mammals, growth hormone-releasing hormone (GHRH) is the most important neuroendocrine factor that stimulates the release of growth hormone (GH) from the anterior pituitary. In nonmammalian vertebrates, however, the previously named GHRH-like peptides were unable to demonstrate robust GH-releasing activities. In this article, we provide evidence that these GHRH-like peptides are homologues of mammalian PACAP-related peptides (PRP). Instead, GHRH peptides encoded in cDNAs isolated from goldfish, zebrafish, and African clawed frog were identified. Moreover, receptors specific for these GHRHs were characterized from goldfish and zebrafish. These GHRHs and GHRH receptors (GHRH-Rs) are phylogenetically and structurally more similar to their mammalian counterparts than the previously named GHRH-like peptides and GHRH-like receptors. Information regarding their chromosomal locations and organization of neighboring genes confirmed that they share the same origins as the mammalian genes. Functionally, the goldfish GHRH dose-dependently activates cAMP production in receptor-transfected CHO cells as well as GH release from goldfish pituitary cells. Tissue distribution studies showed that the goldfish GHRH is expressed almost exclusively in the brain, whereas the goldfish GHRH-R is actively expressed in brain and pituitary. Taken together, these results provide evidence for a previously uncharacterized GHRH-GHRH-R axis in nonmammalian vertebrates. Based on these data, a comprehensive evolutionary scheme for GHRH, PRP-PACAP, and PHI-VIP genes in relation to three rounds of genome duplication early on in vertebrate evolution is proposed. These GHRHs, also found in flounder, Fugu, medaka, stickleback, Tetraodon, and rainbow trout, provide research directions regarding the neuroendocrine control of growth in vertebrates.

KeywordMolecular Evolution Genome Duplication Pituitary Adenylate Cyclase-activating Polypeptide
DOIhttps://doi.org/10.1073/pnas.0611008104
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000244438500020
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Cited Times [WOS]:72   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorBilly K. C. Chow
Affiliation1.Departments of Zoology, University of Hong Kong, Pokfulam Road, Hong Kong, China
2.Departments of Chemistry, University of Hong Kong, Pokfulam Road, Hong Kong, China
3.Institut National de la Santé et de la Recherche Médicale U-413, Laboratory of Cellular and Molecular Neuroendocrinology, European Institute for Peptide Research (Institut Fédératif de Recherches Multidisciplinaires sur les Peptides 23), University of Rouen, 76821 Mont-Saint-Aignan, France
Recommended Citation
GB/T 7714
Leo T. O. Lee,Francis K. Y. Siu,Janice K. V. Tam,et al. Discovery of growth hormone-releasing hormones and receptors in nonmammalian vertebrates[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2007,104(7):2133-2138.
APA Leo T. O. Lee.,Francis K. Y. Siu.,Janice K. V. Tam.,Ivy T. Y. Lau.,Anderson O. L. Wong.,...&Billy K. C. Chow.(2007).Discovery of growth hormone-releasing hormones and receptors in nonmammalian vertebrates.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,104(7),2133-2138.
MLA Leo T. O. Lee,et al."Discovery of growth hormone-releasing hormones and receptors in nonmammalian vertebrates".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 104.7(2007):2133-2138.
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