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GATA-binding protein 4 (GATA-4) and T-cell acute leukemia 1 (TAL1) regulate myogenic differentiation and erythropoietin response via cross-talk with Sirtuin1 (Sirt1).
Li Wang1; Yi Jia1; Heather Rogers1; Yun-Ping Wu2; Suming Huang3; Constance Tom Noguchi1
2012
Source PublicationThe Journal of biological chemistry
ISSN0021-9258
Volume287Issue:36Pages:30157-30169
Abstract

Erythropoietin (EPO), the cytokine required for erythrocyte production, contributes to muscle progenitor cell proliferation and delay myogenic differentiation. However, the underlying mechanism is not yet fully understood. Here, we report that EPO changes the skeletal myogenic regulatory factor expression program and delays differentiation via induction of GATA-4 and the basic helix-loop-helix TAL1 and that knockdown of both factors promotes differentiation. EPO increases the Sirt1 level, a NAD(+)-dependent deacetylase, and also induces the NAD(+)/NADH ratio that further increases Sirt1 activity. Sirt1 knockdown reduced GATA-4 and TAL1 expression, impaired EPO effect on delayed myogenic differentiation, and the Sirt1 knockdown effect was abrogated when combined with overexpression of GATA-4 or TAL1. GATA-4 interacts with Sirt1 and targets Sirt1 to the myogenin promoter and represses myogenin expression, whereas TAL1 inhibits myogenin expression by decreasing MyoD binding to and activation of the myogenin promoter. Sirt1 was found to bind to the GATA-4 promoter to directly regulate GATA-4 expression and GATA-4 binds to the TAL1 promoter to regulate TAL1 expression positively. These data suggest that GATA-4, TAL1, and Sirt1 cross-talk each other to regulate myogenic differentiation and mediate EPO activity during myogenic differentiation with Sirt1 playing a role upstream of GATA-4 and TAL1. Taken together, our findings reveal a novel role for GATA-4 and TAL1 to affect skeletal myogenic differentiation and EPO response via cross-talk with Sirt1.

KeywordErythropoietin Gata Molecular Biology Muscle Transcription Factors Myoblast Proliferation Myogenic Differentiation
DOIhttp://dx.doi.org/10.1074/jbc.M112.376640
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000308579800011
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Cited Times [WOS]:15   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Molecular Medicine Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892-1822
2.Genetics of Development and Disease Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892-1822
3.Department of Biochemistry and Molecular Biology and Shands Cancer Center, University of Florida College of Medicine, Gainesville, Florida 32610
Recommended Citation
GB/T 7714
Li Wang,Yi Jia,Heather Rogers,et al. GATA-binding protein 4 (GATA-4) and T-cell acute leukemia 1 (TAL1) regulate myogenic differentiation and erythropoietin response via cross-talk with Sirtuin1 (Sirt1).[J]. The Journal of biological chemistry,2012,287(36):30157-30169.
APA Li Wang,Yi Jia,Heather Rogers,Yun-Ping Wu,Suming Huang,&Constance Tom Noguchi.(2012).GATA-binding protein 4 (GATA-4) and T-cell acute leukemia 1 (TAL1) regulate myogenic differentiation and erythropoietin response via cross-talk with Sirtuin1 (Sirt1)..The Journal of biological chemistry,287(36),30157-30169.
MLA Li Wang,et al."GATA-binding protein 4 (GATA-4) and T-cell acute leukemia 1 (TAL1) regulate myogenic differentiation and erythropoietin response via cross-talk with Sirtuin1 (Sirt1).".The Journal of biological chemistry 287.36(2012):30157-30169.
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