UM  > Faculty of Health Sciences
SATB1 regulates beta-like globin genes through matrix related nuclear relocation of the cluster
Huan Gong; Zhao Wang; Guo-wei Zhao; Xiang Lv; Gong-hong Wei; Li Wang; De-pei Liu; Chih-chuan Liang
2009
Source PublicationBiochemical and Biophysical Research Communications
ISSN0006-291X
Volume383Issue:1Pages:11-15
Abstract

The nuclear location and relocation of genes play crucial regulatory roles in gene expression. SATB1, a MAR-binding protein, has been found to regulate b-like globin genes through chromatin remodeling. In this study, we generated K562 cells over-expressing wild-type or nuclear matrix targeting sequences (NMTS)-deficient SATB1 and found that like wild-type SATB1, NMTS-deficient SATB1 induces out loop of b-globin cluster from its chromosome territory (CT), while it is unable to associate the cluster with the nuclear matrix as wild-type SATB1 does and had no regulatory functions to the b-globin cluster. Besides, our data showed that the transacting factor occupancies and chromatin modifications at b-globin cluster were differentially affected by wild-type and NMTS-deficient SATB1. These results indicate that SATB1 regulates b-like globin genes at the nuclear level interlaced with chromatin and DNA level, and emphasize the nuclear matrix binding activity of SATB1 to its regulatory function.

KeywordB-globin Cluster Gene Regulation Nuclear Matrix Nuclear Relocation Satb1
DOIhttps://doi.org/10.1016/j.bbrc.2009.03.122
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000265768800003
Fulltext Access
Citation statistics
Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationNational Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), 5 Dong Dan San Tiao, Beijing 100005, PR China
Recommended Citation
GB/T 7714
Huan Gong,Zhao Wang,Guo-wei Zhao,et al. SATB1 regulates beta-like globin genes through matrix related nuclear relocation of the cluster[J]. Biochemical and Biophysical Research Communications,2009,383(1):11-15.
APA Huan Gong.,Zhao Wang.,Guo-wei Zhao.,Xiang Lv.,Gong-hong Wei.,...&Chih-chuan Liang.(2009).SATB1 regulates beta-like globin genes through matrix related nuclear relocation of the cluster.Biochemical and Biophysical Research Communications,383(1),11-15.
MLA Huan Gong,et al."SATB1 regulates beta-like globin genes through matrix related nuclear relocation of the cluster".Biochemical and Biophysical Research Communications 383.1(2009):11-15.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Huan Gong]'s Articles
[Zhao Wang]'s Articles
[Guo-wei Zhao]'s Articles
Baidu academic
Similar articles in Baidu academic
[Huan Gong]'s Articles
[Zhao Wang]'s Articles
[Guo-wei Zhao]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Huan Gong]'s Articles
[Zhao Wang]'s Articles
[Guo-wei Zhao]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.