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Irreversible inhibition of CD13/aminopeptidase N by the anti-angiogenic agent curcumin
Joong Sup Shim1; Jin Hee Kim1; Hyun Young Cho2; Young Na Yum2; Seung Hee Kim2; Hyun-Ju Park3; Bum Sang Shim4; Seung Hoon Choi4; Ho Jeong Kwon1
2003
Source PublicationCHEMISTRY & BIOLOGY
ISSN1074-5521
Volume10Issue:8Pages:695-704
Abstract

CD13/aminopeptidase N (APN) is a membrane-bound, zinc-dependent metalloproteinase that plays a key role in tumor invasion and angiogenesis. Here, we show that curcumin, a phenolic natural product, binds to APN and irreversibly inhibits its activity. The direct interaction between curcumin with APN was confirmed both in vitro and in vivo by surface plasmon resonance analysis and an APN-specific antibody competition assay, respectively. Moreover, curcumin and other known APN inhibitors strongly inhibited APN-positive tumor cell invasion and basic fibroblast growth factor-induced angiogenesis. However, curcumin did not inhibit the invasion of APN-negative tumor cells, suggesting that the antiinvasive activity of curcumin against tumor cells is attributable to the inhibition of APN. Taken together, our study revealed that curcumin is a novel irreversible inhibitor of APN that binds to curcumin resulting in inhibition of angiogenesis.

DOIhttps://doi.org/10.1016/S1074-5521(03)00169-8
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000185031100004
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Cited Times [WOS]:119   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorHo Jeong Kwon
Affiliation1.Department of Bioscience and Biotechnology, Institute of Bioscience, Sejong University, Seoul 143-747, South Korea
2.National Institute of Toxicological Research, Korea Food and Drug Administration, Seoul 122-704, South Korea
3.College of Pharmacy, Sungkyunkwan University, Suwon 440-746, South Korea
4.College of Oriental Medicine, Kyung Hee University, Seoul 130-701, South Korea
Recommended Citation
GB/T 7714
Joong Sup Shim,Jin Hee Kim,Hyun Young Cho,et al. Irreversible inhibition of CD13/aminopeptidase N by the anti-angiogenic agent curcumin[J]. CHEMISTRY & BIOLOGY,2003,10(8):695-704.
APA Joong Sup Shim.,Jin Hee Kim.,Hyun Young Cho.,Young Na Yum.,Seung Hee Kim.,...&Ho Jeong Kwon.(2003).Irreversible inhibition of CD13/aminopeptidase N by the anti-angiogenic agent curcumin.CHEMISTRY & BIOLOGY,10(8),695-704.
MLA Joong Sup Shim,et al."Irreversible inhibition of CD13/aminopeptidase N by the anti-angiogenic agent curcumin".CHEMISTRY & BIOLOGY 10.8(2003):695-704.
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