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Quick identification of kuraridin, a noncytotoxic anti-MRSA (methicillin-resistant Staphylococcus aureus) agent from Sophora flavescens using high-speed counter-current chromatography
Ben Chung-Lap Chan1; Hua Yu1; Chun-Wai Wong1; Sau-Lai Lui1,2; Claude Jolivalt4,5; Carine Ganem-Elbaz4,5; Jean-Marc Paris4,5; Barbara Morleo6; Marc Litaudon6; Clara Bik-San Lau1; Margaret Ip2; Kwok-Pui Fung1; Ping-Chung Leung1; Quan-Bin Han1,3
2012-01
Source PublicationJournal of chromatography B, Analytical Technologies in the Biomedical and Life Sciences
ISSN1570-0232
Volume880Pages:157-162
Abstract

Bacterial resistance to antibiotics has become a serious problem of public health that concerns almost all currently used antibacterial agents and that manifests in all fields of their application. To find more antibacterial agents from natural resources is all the time considered as an important strategy. Sophora flavescens is a popularly used antibacterial herb in Chinese Medicine, from which prenylated flavones were reported as the antibacterial ingredients but with a major concern of toxicity. In our screening on the antibacterial activities of various chemicals of this herb, 18 fractions were obtained from 8 g of 50% ethanol extract on a preparative high-speed counter-current chromatography (HSCCC, 1000 ml). The system of n-hexane/ethyl acetate/methanol/water (1:1:1:1) was used as the two-phase separation solvent. A chalcone named kurariclin was isolated from the best anti-MRSA fraction, together with sophoraflavanone G. a known active ingredient of S. flovescens. Their structures were elucidated by analysis of the NMR spectra. Both compounds exhibited significant anti-MRSA effects, compared to baicalein that is a well known anti-MRSA natural product. More important, kuraridin showed no toxicity on human peripheral blood mononuclear cells (PBMC) at the concentration up to 6411 mu g/ml while sophoraflavanone G inhibited over 50% of cellular activity at 4 mu g/ml or higher concentration. These data suggested that opening of ring A of the prenylated flavones might decrease the toxicity and remain the anti-MRSA effect, from a viewpoint of structure-activity relationship. (C) 2011 Elsevier B.V. All rights reserved.

KeywordMrsa Antibacteria Sophora Flavescens Kuraridin High-speed Counter-current Chromatography
DOIhttp://www.doi.org/10.1016/j.jchromb.2011.11.039
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000299197600022
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Cited Times [WOS]:12   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.State Key Laboratory of Phytochemistry and Plant Resources in West China (CUHK), Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, N. T., Hong Kong SAR, China
2.Department of Microbiology, The Chinese University of Hong Kong, Shatin, N. T., Hong Kong SAR, China
3.School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, China
4.ChimieParisTech, Laboratoire Charles Friedel (LCF), 75005 Paris, France
5.CNRS, UMR 7223, 75005 Paris, France
6.Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles, France
Recommended Citation
GB/T 7714
Ben Chung-Lap Chan,Hua Yu,Chun-Wai Wong,et al. Quick identification of kuraridin, a noncytotoxic anti-MRSA (methicillin-resistant Staphylococcus aureus) agent from Sophora flavescens using high-speed counter-current chromatography[J]. Journal of chromatography B, Analytical Technologies in the Biomedical and Life Sciences,2012,880:157-162.
APA Ben Chung-Lap Chan.,Hua Yu.,Chun-Wai Wong.,Sau-Lai Lui.,Claude Jolivalt.,...&Quan-Bin Han.(2012).Quick identification of kuraridin, a noncytotoxic anti-MRSA (methicillin-resistant Staphylococcus aureus) agent from Sophora flavescens using high-speed counter-current chromatography.Journal of chromatography B, Analytical Technologies in the Biomedical and Life Sciences,880,157-162.
MLA Ben Chung-Lap Chan,et al."Quick identification of kuraridin, a noncytotoxic anti-MRSA (methicillin-resistant Staphylococcus aureus) agent from Sophora flavescens using high-speed counter-current chromatography".Journal of chromatography B, Analytical Technologies in the Biomedical and Life Sciences 880(2012):157-162.
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