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Isolation of Cytoplasmatic Proteins from Cultured Cells for Two-Dimensional Gel Electrophoresis
Wang, Ying; Chiu, Jen-Fu; He, Qing-Yu
2008
Source Publication2D PAGE: Sample Preparation and Fractionation
Author of SourceAnton Posch
PublisherHumana Press
Pages101-112
Abstract

Cytoplasma is the cell interior place between the cellular membrane and the nucleus, where various intracellular activities take place, including energy production, reactive oxygen species (ROS) detoxification, heme synthesis, nitrogen and lipid metabolism, phosphorylation in signal transduction, and cytoskeletal meshwork construction. The rich cytoplasmatic proteins carrying out these intracellular functions are interesting targets for biochemical and molecular biological studies. The relatively recent discipline of proteomics offers a chance to globally analyze the changes in cytoplasmic proteins corresponding to drug treatments or disease conditions, and thus provide target candidates for further biological validation in drug development and biomarker discovery. Isolation of cytoplasmic proteins from cells is a necessary step for high resolution protein separation by two-dimensional gel electrophoresis (2DE) and specific proteomic analysis.

KeywordCytoplasmatic Proteins Protein Isolation Proteomics Sample Preparation Subcellular Fractionation Two-dimensional Gel Electrophoresis
DOIhttps://doi.org/10.1007/978-1-60327-210-0_9
Language英语
ISBN978-1-60327-209-4
Fulltext Access
Citation statistics
Document TypeBook chapter
CollectionInstitute of Chinese Medical Sciences
AffiliationThe University of Hong Kong, Hong Kong, China
Recommended Citation
GB/T 7714
Wang, Ying,Chiu, Jen-Fu,He, Qing-Yu. Isolation of Cytoplasmatic Proteins from Cultured Cells for Two-Dimensional Gel Electrophoresis:Humana Press,2008:101-112.
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