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Importance of Having Low-Density Functional Groups for Generating High-Performance Semiconducting Polymer Dots
Zhang, XJ; Yu, JB; Wu, CF; Jin, YH; Rang, Y; Ye, FM; Chiu, DT
2012
Source PublicationACS NANO
ISSN1936-0851
Volume6Issue:6Pages:5429-5439
Abstract

Semiconducting polymers with low-density side-chain carboxylic acid groups were synthesized to form stable, functionalized, and highly fluorescent polymer dots (Pdots). The influence of the molar fraction of hydrophilic side-chains on Pdot properties and performance was systematically investigated. Our results show that the density of side-chain carboxylic acid groups significantly affects Pdot stability, internal structure, fluorescence brightness, and nonspecific binding in cellular labeling. Fluorescence spectroscopy, single-particle imaging, and a dye-doping method were employed to investigate the fluorescence brightness and the internal structure of the Pdots. The results of these experiments indicate that semiconducting polymers with low density of side-chain functional groups can form stable, compact, and highly bright Pdots as compared to those with high density of hydrophilic side-chains. The functionalized polymer dots were conjugated to streptavidin (SA) by carbodiimide-catalyzed coupling and the Pdot-SA probes effectively and specifically labeled the cancer cell-surface marker Her2 in human breast cancer cells. The carboxylate-functionalized polymer could also be covalently modified with small functional molecules to generate Pdot probes for click chemistry-based bio-orthogonal labeling. This study presents a promising approach forfurther developing functional Pdot probes for biological applications.

DOIhttp://dx.doi.org/10.1021/nn301308w
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science
WOS SubjectChemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinar
WOS IDWOS:000305661300096
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Cited Times [WOS]:75   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationUniv Washington, Dept Chem, Seattle, WA 98195 USA
Recommended Citation
GB/T 7714
Zhang, XJ,Yu, JB,Wu, CF,et al. Importance of Having Low-Density Functional Groups for Generating High-Performance Semiconducting Polymer Dots[J]. ACS NANO,2012,6(6):5429-5439.
APA Zhang, XJ.,Yu, JB.,Wu, CF.,Jin, YH.,Rang, Y.,...&Chiu, DT.(2012).Importance of Having Low-Density Functional Groups for Generating High-Performance Semiconducting Polymer Dots.ACS NANO,6(6),5429-5439.
MLA Zhang, XJ,et al."Importance of Having Low-Density Functional Groups for Generating High-Performance Semiconducting Polymer Dots".ACS NANO 6.6(2012):5429-5439.
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