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Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11
Man Cheng1; Ceci W.L.Chan1; Randy C.F.Cheung1; Rama Kamesh Bikkavilli1; Qi Zhao1; Shannon W.N.Au1; Paul K.S.Chan2; Susanna S.T.Lee1; Gregory Cheng3; Walter K.K.Ho1; Wing-Tai Cheung1
2005
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
Volume338Issue:3Pages:1654-1660
Abstract

Infection of SARS-associated coronavirus (SARS-CoV) induced a strong anti-nucleocapsid (anti-N) antibody response. However, the pathophysiological significance of the anti-N antibodies in SARS pathogenesis is largely unknown. To profile the anti-N antibodies, a phage-displayed scFv library was prepared from mice immunized with heat-inactivated SARS-CoV-infected Vero E6 cell lysate. Specific anti-N scFvs were isolated by panning against a recombinant nucleocapsid protein and reactivity was confirmed with phage-ELISA. Sequence analysis indicated that two of the isolated anti-N scFv clones were identical and displayed a high homology with an scFv specific for interleukin 11 (IL-11), an anti-inflammatory cytokine derived from bone marrow stroma cells. In a neutralization assay, IL-11-induced STAT 3 phosphorylation in rat intestinal epithelial IEC-18 cells was completely suppressed by the anti-N scFv clone L9N01.

KeywordAntibody Interleukin 11 Nucleocapsid Protein Phage-display Sars-coronavirus Scfv
DOIhttps://doi.org/10.1016/j.bbrc.2005.10.088
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000233710100047
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Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorWing-Tai Cheung
Affiliation1.Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
2.Department of Microbiology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
3.Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
Recommended Citation
GB/T 7714
Man Cheng,Ceci W.L.Chan,Randy C.F.Cheung,et al. Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,338(3):1654-1660.
APA Man Cheng.,Ceci W.L.Chan.,Randy C.F.Cheung.,Rama Kamesh Bikkavilli.,Qi Zhao.,...&Wing-Tai Cheung.(2005).Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,338(3),1654-1660.
MLA Man Cheng,et al."Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 338.3(2005):1654-1660.
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