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Jarid2 and PRC2, partners in regulating gene expression
Gang Li1,2; Raphael Margueron2; Manching Ku1,3,4; Pierre Chambon5; Bradley E. Bernstein1,3,4; Danny Reinberg1,2
2010
Source PublicationGENES & DEVELOPMENT
ISSN0890-9369
Volume24Issue:4Pages:368-380
Abstract

The Polycomb group proteins foster gene repression profiles required for proper development and unimpaired adulthood, and comprise the components of the Polycomb-Repressive Complex 2 (PRC2) including the histone H3 Lys 27 (H3K27) methyltransferase Ezh2. How mammalian PRC2 accesses chromatin is unclear. We found that Jarid2 associates with PRC2 and stimulates its enzymatic activity in vitro. Jarid2 contains a Jumonji C domain, but is devoid of detectable histone demethylase activity. Instead, its artificial recruitment to a promoter in vivo resulted in corecruitment of PRC2 with resultant increased levels of di- and trimethylation of H3K27 (H3K27me2/3). Jarid2 colocalizes with Ezh2 and MTF2, a homolog of Drosophila Pcl, at endogenous genes in embryonic stem (ES) cells. Jarid2 can bind DNA and its recruitment in ES cells is interdependent with that of PRC2, as Jarid2 knockdown reduced PRC2 at its target promoters, and ES cells devoid of the PRC2 component EED are deficient in Jarid2 promoter access. In addition to the well-documented defects in embryonic viability upon down-regulation of Jarid2, ES cell differentiation is impaired, as is Oct4 silencing.

KeywordPolycomb Chromatin Histone Methylation Jarid2
DOIhttps://doi.org/10.1101/gad.1886410
Indexed BySCI
Language英语
WOS Research AreaCell Biology ; Developmental Biology ; Genetics & Heredity
WOS SubjectCell Biology ; Developmental Biology ; Genetics & Heredity
WOS IDWOS:000274577100006
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Cited Times [WOS]:313   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorDanny Reinberg
Affiliation1.Howard Hughes Medical Institute, New York University Medical School, New York, New York 10016, USA
2.Department of Biochemistry, New York University Medical School, New York, New York 10016, USA
3.Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, Massachusetts 02142, USA
4.Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
5.Department of Functional Genomics, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Institut Clinique de la Souris (ICS), CNRS/INSERM/Université de Strasbourg, BP10142, 67404 Illkirch, France
Recommended Citation
GB/T 7714
Gang Li,Raphael Margueron,Manching Ku,et al. Jarid2 and PRC2, partners in regulating gene expression[J]. GENES & DEVELOPMENT,2010,24(4):368-380.
APA Gang Li,Raphael Margueron,Manching Ku,Pierre Chambon,Bradley E. Bernstein,&Danny Reinberg.(2010).Jarid2 and PRC2, partners in regulating gene expression.GENES & DEVELOPMENT,24(4),368-380.
MLA Gang Li,et al."Jarid2 and PRC2, partners in regulating gene expression".GENES & DEVELOPMENT 24.4(2010):368-380.
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