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Reversible Photoswitching of Spiropyran-Conjugated Semiconducting Polymer Dots
Chan, YH; Gallina, ME; Zhang, XJ; Wu, IC; Jin, YH; Sun, W; Chiu, DT
2012
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume84Issue:21Pages:9431-9438
Abstract

Semiconducting polymer dots (Pdots) recently have emerged as a new class of ultrabright fluorescent probes with promising applications in biological detection and imaging. We developed photoswitchable Pdots by conjugating photochromic spiropyran molecules onto poly[9,9-dioctylfluorenyl-2,7-diyl)-co-1,4-benzo-{2,1'-3}-thiadiazole)] (PFBT). The modulation of fluorescence was achieved by ultraviolet irradiation, which converted spiropyran into its visible-absorbing merocyanine form. The merocyanine efficiently quenched the fluorescence of PFBT via Forster resonance energy transfer (FRET). We then reversed the quenching by subsequent irradiation with visible light to get back the fluorescence of PFBT. This FRET-based photomodulation of Pdot fluorescence could be repeated multiple times. We next conjugated biomolecules onto the surface ofthese photoswitchable Pdots and demonstrated their specific cellular and subcellular labeling to different types of cells without any noticeable nonspecific binding. We anticipate these photoswitchable and biocompatible Pdots will be useful in developing bioimaging techniques in the future.

DOIhttp://dx.doi.org/10.1021/ac302245t
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000310664600078
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Cited Times [WOS]:56   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationUniv Washington, Dept Chem, Seattle, WA 98195 USA
Recommended Citation
GB/T 7714
Chan, YH,Gallina, ME,Zhang, XJ,et al. Reversible Photoswitching of Spiropyran-Conjugated Semiconducting Polymer Dots[J]. ANALYTICAL CHEMISTRY,2012,84(21):9431-9438.
APA Chan, YH.,Gallina, ME.,Zhang, XJ.,Wu, IC.,Jin, YH.,...&Chiu, DT.(2012).Reversible Photoswitching of Spiropyran-Conjugated Semiconducting Polymer Dots.ANALYTICAL CHEMISTRY,84(21),9431-9438.
MLA Chan, YH,et al."Reversible Photoswitching of Spiropyran-Conjugated Semiconducting Polymer Dots".ANALYTICAL CHEMISTRY 84.21(2012):9431-9438.
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