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A facile “click” reaction to fabricate a FRET-based ratiometric fluorescent Cu2+ probe
Hu, ZJ1,2; Hu, JW1; Cui, Y1; Wang, GN2; Zhang, XJ2; Uvdal, K2; Gao, HW1
2014
Source PublicationJOURNAL OF MATERIALS CHEMISTRY B
ISSN2050-7518
Volume2Issue:28Pages:4467-4472
Abstract

A facile one-step Cu(I)-catalyzed “click” reaction, between a dansyl-azide and a propargyl-substituted rhodamine B hydrazide, is employed to fabricate a novel FRET ratiometric “off–on” fluorescent probe. The sensitive emission of the donor, a dansyl group, overlaps perfectly with the absorption of the acceptor, xanthene in the open-ring rhodamine. The proposed probe shows high selectivity towards Cu2+. The ratio of emission intensities at 568 and 540 nm (I568/I540) exhibits a drastic 28-fold enhancement upon addition of Cu2+. The probe shows an excellent linear relationship between emission ratios and the concentrations of Cu2+ from 10 to 50 μM, with a detection limit (S/N = 3) of 0.12 μM. The preliminary cellular studies demonstrated that the probe is cell membrane permeable and could be applied for ratiometric fluorescence imaging of intracellular Cu2+with almost no cytotoxicity. The ingenuity of the probe design is to construct a FRET donor–acceptor interconnector and a selective receptor simultaneously by “click” reaction. The strategy was verified to have great potential for developing novel FRET probes for Cu2+.

DOIhttp://dx.doi.org/10.1039/c4tb00441h
Indexed BySCI
Language英语
WOS Research AreaMaterials Science
WOS SubjectMaterials Science, bioMaterials
WOS IDWOS:000338646000009
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Cited Times [WOS]:54   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Tongji Univ, State Key Lab Pollut Control & Resource Reuse, Shanghai 200092, Peoples R China
2.Linkoping Univ, IFM, Div Mol Surface Phys & Nanosci, S-58183 Linkoping, Sweden
Recommended Citation
GB/T 7714
Hu, ZJ,Hu, JW,Cui, Y,et al. A facile “click” reaction to fabricate a FRET-based ratiometric fluorescent Cu2+ probe[J]. JOURNAL OF MATERIALS CHEMISTRY B,2014,2(28):4467-4472.
APA Hu, ZJ.,Hu, JW.,Cui, Y.,Wang, GN.,Zhang, XJ.,...&Gao, HW.(2014).A facile “click” reaction to fabricate a FRET-based ratiometric fluorescent Cu2+ probe.JOURNAL OF MATERIALS CHEMISTRY B,2(28),4467-4472.
MLA Hu, ZJ,et al."A facile “click” reaction to fabricate a FRET-based ratiometric fluorescent Cu2+ probe".JOURNAL OF MATERIALS CHEMISTRY B 2.28(2014):4467-4472.
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