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A rhodamine-based fluorescent probe for Hg2+ and its application for biological visualization
Hu, JW1; Hu, ZJ1,2; Cui, Y1; Zhang, XJ2; Gao, HW1; Uvdal, K2
2014
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
Volume203Pages:452-458
Abstract

A new visible light excitable fluorescent probe (1) is synthesized by appending a hydroxymethyl-pyridine to rhodamine B hydrazide. The probe displays very specific Hg2+-induced colour change and fluorescent enhancement in the aqueous systems. The "turn-on" response of fluorescence is based on a binding-induced ring-opening process from the spirolactam (nonfluorescent) to acyclic xanthene (fluorescent) in rhodamine B. The coordinating atoms O-center dot-N-N-O-center dot from the hydroxymethyl-pyridine and rhodamine B hydrazide play dominant role in the formation of a complex with 1:1 stoichiometry of Hg2+ to 1. It exhibits a linear response in the range of 0.1-5 mu M with the limit of detection (LOD) of 15.7 nM (3 sigma/slope), while the calculated value of the association constant of Hg2+/1 is 0.70 x 10(5) M-1. Furthermore, confocal microscopy imaging experiment demonstrates the probe 1 can be applied as afluorescent probe for visualization of Hg2+ in living HeLa cells. (C) 2014 Elsevier B.V. All rights reserved.

KeywordMercury Fluorescent Imaging Fluorescence Probe Rhodamine
DOIhttp://dx.doi.org/10.1016/j.snb.2014.06.104
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000341455400059
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Cited Times [WOS]:30   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Tongji Univ, Coll Environm Sci & Engn, State Key Lab Pollut Control & Resource Reuse, Shanghai 200092, Peoples R China
2.Linkoping Univ, Dept Phys Chem & Biol, Div Mol Surface Phys & Nanosci, S-58183 Linkoping, Sweden
Recommended Citation
GB/T 7714
Hu, JW,Hu, ZJ,Cui, Y,et al. A rhodamine-based fluorescent probe for Hg2+ and its application for biological visualization[J]. SENSORS AND ACTUATORS B-CHEMICAL,2014,203:452-458.
APA Hu, JW,Hu, ZJ,Cui, Y,Zhang, XJ,Gao, HW,&Uvdal, K.(2014).A rhodamine-based fluorescent probe for Hg2+ and its application for biological visualization.SENSORS AND ACTUATORS B-CHEMICAL,203,452-458.
MLA Hu, JW,et al."A rhodamine-based fluorescent probe for Hg2+ and its application for biological visualization".SENSORS AND ACTUATORS B-CHEMICAL 203(2014):452-458.
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