UM  > 健康科學學院
Genetic Analysis of Anti-Amoebae and Anti-Bacterial Activities of the Type VI Secretion System in Vibrio cholera
Jun Zheng; Brian Ho; John J. Mekalanos
2011
Source PublicationPLoS ONE
ISSN1932-6203
Volume6Issue:8Pages:1-12
Abstract

A type VI secretion system (T6SS) was recently shown to be required for full virulence of Vibrio cholerae O37 serogroup strain V52. In this study, we systematically mutagenized each individual gene in T6SS locus and characterized their functions based on expression and secretion of the hemolysin co-regulated protein (Hcp), virulence towards amoebae of Dictyostelium discoideum and killing of Escherichia coli bacterial cells. We group the 17 proteins characterized in the T6SS locus into four categories: twelve (VipA, VipB, VCA0109–VCA0115, ClpV, VCA0119, and VasK) are essential for Hcp secretion and bacterial virulence, and thus likely function as structural components of the apparatus; two (VasH and VCA0122) are regulators that are required for T6SS gene expression and virulence; another two, VCA0121 and valine-glycine repeat protein G 3 (VgrG-3), are not essential for Hcp expression, secretion or bacterial virulence, and their functions are unknown; the last group is represented by VCA0118, which is not required for Hcp expression or secretion but still plays a role in both amoebae and bacterial killing and may therefore be an effector protein. We also showed that the clpV gene product is required for Dictyostelium virulence but is less important for killing E. coli. In addition, one vgrG gene (vgrG-2) outside of the T6SS gene cluster was required for bacterial killing but another (vgrG-1) was not. However, a bacterial killing defect was observed when vgrG-1 and vgrG-3 were both deleted. Several genes encoded in the same putative operon as vgrG-1 and vgrG-2 also contribute to virulence toward Dictyostelium but have a smaller effect on bacterial killing. Our results provide new insights into the functional requirements of V. cholerae’s T6SS in the context of secretion as well as killing of bacterial and eukaryotic phagocytic cells.

DOIhttps://doi.org/10.1371/journal.pone.0023876
Indexed By其他
Language英语
Fulltext Access
Citation statistics
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorJohn J. Mekalanos
AffiliationDepartment of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
Recommended Citation
GB/T 7714
Jun Zheng,Brian Ho,John J. Mekalanos. Genetic Analysis of Anti-Amoebae and Anti-Bacterial Activities of the Type VI Secretion System in Vibrio cholera[J]. PLoS ONE,2011,6(8):1-12.
APA Jun Zheng,Brian Ho,&John J. Mekalanos.(2011).Genetic Analysis of Anti-Amoebae and Anti-Bacterial Activities of the Type VI Secretion System in Vibrio cholera.PLoS ONE,6(8),1-12.
MLA Jun Zheng,et al."Genetic Analysis of Anti-Amoebae and Anti-Bacterial Activities of the Type VI Secretion System in Vibrio cholera".PLoS ONE 6.8(2011):1-12.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jun Zheng]'s Articles
[Brian Ho]'s Articles
[John J. Mekalanos]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jun Zheng]'s Articles
[Brian Ho]'s Articles
[John J. Mekalanos]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jun Zheng]'s Articles
[Brian Ho]'s Articles
[John J. Mekalanos]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.