UM
Poly(ethylene glycol)-poly-l-glutamate complexed with polyethyleneimine-polyglycine for highly efficient gene delivery in vitro and in vivo
Guo, Zhaopei1,2; Lin, Lin2; Chen, Jie2; Zhou, Xingzhi1; Chan, Hon Fai3; Chen, Xuesi2; Tian, Huayu2; Chen, Meiwan1
2018-11
Source PublicationBIOMATERIALS SCIENCE
ISSN2047-4830
Volume6Issue:11Pages:3053-3062
AbstractTo improve gene transfection efficiency in vitro and in vivo, we first grafted polyglycine (PGly) onto branched polyethyleneimine (PEI) (molecular weight M-W 25 kDa) to obtain a novel gene vector PGly-PEI (PPG), before complexing it with poly(ethylene glycol)-poly-l-glutamate (PEG-PLG) for adjusting the charge density of PEI. We named the resulting nano-polyplex PG/PPG. The PPGs demonstrated improved gene transfection efficiency after grafting with polyglycine, and the efficiency could be further increased with the introduction of PEG-PLG. The PG/PPG exhibited not only excellent DNA and siRNA transfection capacity in various cell lines including HeLa, CHO, HepG2, MCF7 and Huh7, but also reduced cytotoxicity compared with PEI. Besides, PG/PPG induced a higher degree of cell apoptosis when mixed genes of pKH3-rev-casp-3 plasmid and siBcl2 were used rather than either of them. Furthermore, PG/PPG was highly resistant to serum, which led to enhanced in vivo red fluorescent protein (RFP) expression in HeLa tumors. These results suggested that PG/PPG is a promising gene carrier for highly efficient gene delivery.
DOI10.1039/c8bm00503f
URLView the original
Indexed BySCI
Language英语
WOS Research AreaMaterials Science
WOS SubjectMaterials Science, Biomaterials
WOS IDWOS:000448822800024
PublisherROYAL SOC CHEMISTRY
Fulltext Access
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Taipa 9990786, Macao, Peoples R China;
2.Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Polymer Ecomat, Changchun 130022, Jilin, Peoples R China;
3.Chinese Univ Hong Kong, Sch Biomed Sci, Inst Tissue Engn & Regenerat Med, Hong Kong, Hong Kong, Peoples R China
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Guo, Zhaopei,Lin, Lin,Chen, Jie,et al. Poly(ethylene glycol)-poly-l-glutamate complexed with polyethyleneimine-polyglycine for highly efficient gene delivery in vitro and in vivo[J]. BIOMATERIALS SCIENCE,2018,6(11):3053-3062.
APA Guo, Zhaopei.,Lin, Lin.,Chen, Jie.,Zhou, Xingzhi.,Chan, Hon Fai.,...&Chen, Meiwan.(2018).Poly(ethylene glycol)-poly-l-glutamate complexed with polyethyleneimine-polyglycine for highly efficient gene delivery in vitro and in vivo.BIOMATERIALS SCIENCE,6(11),3053-3062.
MLA Guo, Zhaopei,et al."Poly(ethylene glycol)-poly-l-glutamate complexed with polyethyleneimine-polyglycine for highly efficient gene delivery in vitro and in vivo".BIOMATERIALS SCIENCE 6.11(2018):3053-3062.
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