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A long-lived peptide-conjugated iridium(iii) complex as a luminescent probe and inhibitor of the cell migration mediator, formyl peptide receptor 2
Vellaisamy, Kasipandi1; Li, Guodong2; Wang, Wanhe1; Leung, Chung-Hang2; Ma, Dik-Lung1
2018-11-21
Source PublicationCHEMICAL SCIENCE
ISSN2041-6520
Volume9Issue:43
AbstractFormyl peptide receptors play important biological and therapeutic roles in wound repair and inflammatory diseases. In this work, we present a luminescent iridium(iii) complex (6) conjugated with the peptide agonist WKYMVm as a luminescent formyl peptide receptor 2 (FPR2) imaging probe in living cells. Complex 6 displayed ideal cell imaging characteristics, high photostability and low cytotoxicity. Competition assays with a known FPR2 antagonist, WRW4, and siRNA knockdown experiments both revealed that complex 6 selectively targeted FPR2 in living HUVEC cells. Moreover, complex 6 regulated FPR2 signalling in HUVEC cells as shown using a mechanical scratch assay. Finally, complex 6 reduced epithelial cell migration capacity and inhibited lipoxin A4 (LXA4)-triggered cell migration in HUVEC cells, demonstrating the ability of this complex to inhibit FPR2 in living cells. To our knowledge, this is the first long-lived probe for imaging FPR2 in living cells.
DOI10.1039/c8sc02733a
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000449701000001
PublisherROYAL SOC CHEMISTRY
Fulltext Access
Citation statistics
Cited Times [WOS]:29   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Hong Kong Baptist Univ, Dept Chem, Kowloon Tong, Hong Kong, Peoples R China;
2.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau, Peoples R China
Recommended Citation
GB/T 7714
Vellaisamy, Kasipandi,Li, Guodong,Wang, Wanhe,et al. A long-lived peptide-conjugated iridium(iii) complex as a luminescent probe and inhibitor of the cell migration mediator, formyl peptide receptor 2[J]. CHEMICAL SCIENCE,2018,9(43).
APA Vellaisamy, Kasipandi,Li, Guodong,Wang, Wanhe,Leung, Chung-Hang,&Ma, Dik-Lung.(2018).A long-lived peptide-conjugated iridium(iii) complex as a luminescent probe and inhibitor of the cell migration mediator, formyl peptide receptor 2.CHEMICAL SCIENCE,9(43).
MLA Vellaisamy, Kasipandi,et al."A long-lived peptide-conjugated iridium(iii) complex as a luminescent probe and inhibitor of the cell migration mediator, formyl peptide receptor 2".CHEMICAL SCIENCE 9.43(2018).
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