UM
The epigenetically downregulated factor CYGB suppresses breast cancer through inhibition of glucose metabolism
Feng, Yixiao1; Wu, Mingjun2; Li, Shuman3; He, Xiaoqian1; Tang, Jun1; Peng, Weiyan1; Zeng, Beilei1; Deng, Chuxia4; Ren, Guosheng1; Xiang, Tingxiu1
2018-12-13
Source PublicationJOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
ISSN1756-9966
Volume37
AbstractBackgroundRecent studies suggested the globin family member cytoglobin (CYGB) as a potential tumor suppressor; however, the mechanism by which CYGB suppresses cancer is elusive. We investigated the role and mechanism of CYGB in suppressing breast cancer.MethodsCYGB expression was examined by reverse transcription PCR, quantitative reverse transcription PCR and open database analysis. Promoter methylation was examined by methylation-specific PCR. Metabolomics and proteomics were analyzed by gas chromatography-mass spectrometry and isobaric tags for relative and absolute quantitation, respectively. The effects and mechanisms of ectopic CYGB expression in breast cancer cells were assessed with molecular biological and cellular approaches in vitro and with a xenograft tumor model in nude mice.ResultsCYGB expression was downregulated in breast cancer tissues and cell lines, which was associated with promoter methylation. Ectopic CYGB expression suppressed proliferation, migration, invasion and induced apoptosis in breast cancer cell lines MCF7 (p53WT) and MB231 (p53mt) in vitro, and inhibited xenograft tumor growth in vivo. By proteomics and metabolomics analysis, glucose metabolism was found to be one of the main pathways suppressed by CYGB. The CYGB-expressing cells had lower ATP and compromised glycolysis. Additionally, CYGB suppressed key glucose metabolism factors including GLUT1 and HXK2 in p53-dependent and -independent manners. Restoration of GLUT1 or HXK2 expression attenuated CYGB-mediated proliferation suppression and apoptosis induction.ConclusionsCYGB is a potential tumor suppressor in breast cancer that is epigenetically suppressed. The results for the first time suggest that CYGB suppresses breast cancer through inhibiting glucose metabolism, which could be exploited for breast cancer prevention and therapy.
KeywordCYGB GLUT1 HXK2 p53 Breast cancer Metabolism Glycolysis
DOI10.1186/s13046-018-0979-9
URLView the original
Indexed BySCI
Language英语
WOS Research AreaOncology
WOS SubjectOncology
WOS IDWOS:000453284300001
PublisherBMC
Fulltext Access
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Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Chongqing Med Univ, Chongqing Key Lab Mol Oncol & Epigenet, Affiliated Hosp 1, Chongqing, Peoples R China;
2.Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China;
3.Chongqing Med Univ, Dept Oncol, Affiliated Hosp 2, Chongqing, Peoples R China;
4.Univ Macau, Fac Hlth Sci, Taipa, Macau, Peoples R China
Recommended Citation
GB/T 7714
Feng, Yixiao,Wu, Mingjun,Li, Shuman,et al. The epigenetically downregulated factor CYGB suppresses breast cancer through inhibition of glucose metabolism[J]. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH,2018,37.
APA Feng, Yixiao.,Wu, Mingjun.,Li, Shuman.,He, Xiaoqian.,Tang, Jun.,...&Xiang, Tingxiu.(2018).The epigenetically downregulated factor CYGB suppresses breast cancer through inhibition of glucose metabolism.JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH,37.
MLA Feng, Yixiao,et al."The epigenetically downregulated factor CYGB suppresses breast cancer through inhibition of glucose metabolism".JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH 37(2018).
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