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The influence of physiological environment on the targeting effect of aptamer-guided gold nanoparticles
Ding, Ding1,2,8; Zhang, Yinling1,2; Sykes, Edward A.3; Chen, Long4; Chen, Zhuo1,2; Tan, Weihong1,2,5,6,7
2019-01
Source PublicationNANO RESEARCH
ISSN1998-0124
Volume12Issue:1Pages:129-135
AbstractAptamer guided nanomedicine shows great promise in targeted cancer therapies. However the loss of targeting capacity during in vivo or clinical trials has largely hindered its popularity and there are no systematic studies to elucidate the causes. Herein, we investigated such loss of targeting capacity by examining how the physiological milieu affected targeting effect. Aptamer functionalized gold nanoparticle (AuNP) was chosen as the model and exposed to human blood serum that is used to mimic physiological milieu. Dynamic light scattering (DLS), flow cytometry and label-free liquid chromatography tandem mass spectrometry (LC-MS/MS) were employed to determine variations of NPs' surface chemistry and biological identities changes after serum exposure. Results showed that the targeting ability loss was caused by protein corona blocking, replacement and enzymatic cleavage of surface aptamer targeting ligands. Noteworthy, the aggregation issue is critical for the smaller NPs. Analysis of the protein corona profile indicated the accumulation of immune-related proteins on the surface of aptamer-conjugated NPs, which could induce immune response, resulting in rapid clearance of NPs.
Keywordtargeting effect aptamer nanomedicine physiological environment
DOI10.1007/s12274-018-2191-9
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS SubjectChemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
WOS IDWOS:000453629900017
PublisherTSINGHUA UNIV PRESS
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Citation statistics
Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Mol Sci & Biomed Lab MBL, Changsha 410082, Hunan, Peoples R China;
2.Hunan Univ, Coll Life Sci, Changsha 410082, Hunan, Peoples R China;
3.Queens Univ, Sch Med, 81 Barrie St, Kingston, ON K7L 3N6, Canada;
4.Univ Macau, Fac Sci & Technol, Ave Univ, Taipa 999078, Macao, Peoples R China;
5.Univ Florida, UF Genet Inst, UF Hlth Canc Ctr, Dept Chem,Dept Physiol,Ctr Res Bio Nano Interface, Gainesville, FL 32611 USA;
6.Univ Florida, UF Genet Inst, UF Hlth Canc Ctr, Dept Chem,Dept Funct Genom,Ctr Res Bio Nano Inter, Gainesville, FL 32611 USA;
7.Univ Florida, McKnight Brain Inst, Gainesville, FL 32611 USA;
8.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Inst Mol Med, Shanghai 200240, Peoples R China
Recommended Citation
GB/T 7714
Ding, Ding,Zhang, Yinling,Sykes, Edward A.,et al. The influence of physiological environment on the targeting effect of aptamer-guided gold nanoparticles[J]. NANO RESEARCH,2019,12(1):129-135.
APA Ding, Ding,Zhang, Yinling,Sykes, Edward A.,Chen, Long,Chen, Zhuo,&Tan, Weihong.(2019).The influence of physiological environment on the targeting effect of aptamer-guided gold nanoparticles.NANO RESEARCH,12(1),129-135.
MLA Ding, Ding,et al."The influence of physiological environment on the targeting effect of aptamer-guided gold nanoparticles".NANO RESEARCH 12.1(2019):129-135.
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