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Comparison of the toxicities, activities and chemical profiles of raw and processed Xanthii Fructus
Su T.3; Cheng B.C.-Y.3; Fu X.-Q.3; Li T.3; Guo H.3; Cao H.-H.3; Kwan H.-Y.3; Tse A.K.-W.3; Yu H.3; Cao H.1; Yu Z.-L.3
2016-01-22
Source PublicationBMC Complementary and Alternative Medicine
ISSN14726882
Volume16Issue:1
AbstractBackground: Although toxic, the Chinese medicinal herb Xanthii Fructus (XF) is commonly used to treat traditional Chinese medicine (TCM) symptoms that resemble cold, sinusitis and arthritis. According to TCM theory, stir-baking (a processing method) can reduce the toxicity and enhance the efficacy of XF. Methods: Cytotoxicities of raw XF and processed XF (stir-baked XF, SBXF) were determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay in normal liver derived MIHA cells. Nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) mRNA expression were measured by the Griess reagent and quantitative real-time PCR, respectively. The chemical profiles of XF and SBXF were compared using an established ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS) method. Results: SBXF was less toxic than XF in MIHA cells. Both XF and SBXF had anti-inflammatory effects as demonstrated by their abilities to reduce nitric oxide production as well as inducible nitric oxide synthase mRNA expression in lipopolysaccharide-stimulated RAW 264.7 macrophages. Interestingly, the anti-inflammatory effects of SBXF were more potent than that of XF. By comparing the chemical profiles, we found that seven peaks were lower, while nine other peaks were higher in SBXF than in XF. Eleven compounds including carboxyatractyloside, atractyloside and chlorogenic acid corresponding to eleven individual changed peaks were tentatively identified by matching with empirical molecular formulae and mass fragments, as well as literature data. Conclusion: Our study showed that stir-baking significantly reduced the cytotoxicity and enhanced the anti-inflammatory effects of XF; moreover, with a developed ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry method we differentiated XF and SBXF by their chemical profiles. Further studies are warranted to establish the relationship between the alteration of chemical profiles and the changes of medicinal properties caused by stir-baking.
KeywordAnti-inflammation Cytotoxicity Stir-baking UPLC/Q-TOF-MS Xanthii fructus
DOI10.1186/s12906-016-0994-3
URLView the original
Language英語
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Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.National Engineering Research Center for Modernization of Traditional Chinese Medicine
2.HKBU Institute of Research and Continuing Education
3.Hong Kong Baptist University
Recommended Citation
GB/T 7714
Su T.,Cheng B.C.-Y.,Fu X.-Q.,et al. Comparison of the toxicities, activities and chemical profiles of raw and processed Xanthii Fructus[J]. BMC Complementary and Alternative Medicine,2016,16(1).
APA Su T..,Cheng B.C.-Y..,Fu X.-Q..,Li T..,Guo H..,...&Yu Z.-L..(2016).Comparison of the toxicities, activities and chemical profiles of raw and processed Xanthii Fructus.BMC Complementary and Alternative Medicine,16(1).
MLA Su T.,et al."Comparison of the toxicities, activities and chemical profiles of raw and processed Xanthii Fructus".BMC Complementary and Alternative Medicine 16.1(2016).
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