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Structural and biochemical studies of the distinct activity profiles of Rai1 enzymes
Vivien Ya-Fan Wang1; Xinfu Jiao2; Megerditch Kiledjian2; Liang Tong1
2015
Source PublicationNUCLEIC ACIDS RESEARCH
ISSN0305-1048
Volume43Issue:13Pages:6596-6606
Abstract

Recent studies showed that Rai1 and its homologs are a crucial component of the mRNA 5'-end capping quality control mechanism. They can possess RNA 5'-end pyrophosphohydrolase (PPH), decapping, and 5'-3' exonuclease (toward 5' monophosphate RNA) activities, which help to degrade mRNAs with incomplete 5'-end capping. A single active site in the enzyme supports these apparently distinct activities. However, each Rai1 protein studied so far has a unique set of activities, and the molecular basis for these differences are not known. Here, we have characterized the highly diverse activity profiles of Rai1 homologs from a collection of fungal organisms and identified a new activity for these enzymes, 5'-end triphosphonucleotide hydrolase (TPH) instead of PPH activity. Crystal structures of two of these enzymes bound to RNA oligonucleotides reveal differences in the RNA binding modes. Structure-based mutations of these enzymes, changing residues that contact the RNA but are poorly conserved, have substantial effects on their activity, providing a framework to begin to understand the molecular basis for the different activity profiles.

DOIhttps://doi.org/10.1093/nar/gkv620
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000359776500039
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Cited Times [WOS]:6   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorLiang Tong
Affiliation1.Department of Biological Sciences, Columbia University, New York, NY 10027, USA
2.Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854, USA
Recommended Citation
GB/T 7714
Vivien Ya-Fan Wang,Xinfu Jiao,Megerditch Kiledjian,et al. Structural and biochemical studies of the distinct activity profiles of Rai1 enzymes[J]. NUCLEIC ACIDS RESEARCH,2015,43(13):6596-6606.
APA Vivien Ya-Fan Wang,Xinfu Jiao,Megerditch Kiledjian,&Liang Tong.(2015).Structural and biochemical studies of the distinct activity profiles of Rai1 enzymes.NUCLEIC ACIDS RESEARCH,43(13),6596-6606.
MLA Vivien Ya-Fan Wang,et al."Structural and biochemical studies of the distinct activity profiles of Rai1 enzymes".NUCLEIC ACIDS RESEARCH 43.13(2015):6596-6606.
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