UM
Last intron of the chemokine-like factor gene contains a putative promoter for the downstream CKLF super family member 1 gene
Xu M.; Han W.; Qian M.; Ma X.; Ding P.; Wang Y.; Xia D.; Rui M.; Wang L.; Zhang Y.; Ma D.
2004-01-02
Source PublicationBiochemical and Biophysical Research Communications
ISSN0006291X
Volume313Issue:1Pages:135-141
AbstractThe genes for chemokine-like factor (CKLF) and four chemokine-like factor super family members (CKLFSF1-4) are tightly linked on chromosome 16, with only 325bp separating CKLF and CKLFSF1. We used Northern blotting and RT-PCR to show that these two genes are expressed independently of one another. We then used a novel computational promoter prediction method based on the interaction among transcription factor binding sites (TFBSs) to identify a putative promoter region for the CKLFSF1 gene. Our method predicted a promoter region in the last intron of the upstream gene, CKLF. We PCR amplified the predicted promoter region and used a luciferase assay to show that the region was able to drive the luciferase gene. DNA decoy experiments indicated that 214bp fragment neighboring the TATA box markedly inhibited CKLFSF1 gene expression. Sequence analysis of the region revealed a typical TATA box (TATATAA) and multiple potential transcription factor binding sites, providing further evidence for this being a functional promoter for CKLFSF1. This work provides the first evidence of a promoter from one gene located in an intron of another. © 2003 Elsevier Inc. All rights reserved.
KeywordChemokine-like factor Chemokine-like factor super family Intron Luciferase assay
DOI10.1016/j.bbrc.2003.11.100
URLView the original
Language英語
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
AffiliationPeking University
Recommended Citation
GB/T 7714
Xu M.,Han W.,Qian M.,et al. Last intron of the chemokine-like factor gene contains a putative promoter for the downstream CKLF super family member 1 gene[J]. Biochemical and Biophysical Research Communications,2004,313(1):135-141.
APA Xu M..,Han W..,Qian M..,Ma X..,Ding P..,...&Ma D..(2004).Last intron of the chemokine-like factor gene contains a putative promoter for the downstream CKLF super family member 1 gene.Biochemical and Biophysical Research Communications,313(1),135-141.
MLA Xu M.,et al."Last intron of the chemokine-like factor gene contains a putative promoter for the downstream CKLF super family member 1 gene".Biochemical and Biophysical Research Communications 313.1(2004):135-141.
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