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Intranasal delivery of chitosan-DNA vaccine generates mucosal SIgA and anti-CVB3 protection
Xu W.2; Shen Y.2; Jiang Z.2; Wang Y.2; Chu Y.2; Xiong S.2
2004-09-09
Source PublicationVaccine
ISSN0264410X
Volume22Issue:27-28Pages:3603-3612
AbstractCoxsackievirus B3 infections are common causes of acute and chronic myocarditis with no effective prophylactic treatment available. We describe here a prophylactic strategy using chitosan-DNA intranasal immunization to induce CVB3 specific immune responses. Intranasal administration with chitosan-DNA complex prepared by votexing DNA with chitosan, a natural mucus absorption enhancer, resulted in transgenic DNA expression in mouse nasopharynx. Mice immunized with chitosan-DNA (pcDNA3-VP1) encoding VP1, major structural protein of CVB3, produced much higher levels of serum IgG and mucosal secretory IgA compared to mice treated with pcDNA3-VP1 or pcDNA3. Increased virus-specific cytotoxic activity of spleen cells derived from chitosan-DNA vaccinated mice was also determined. Chitosan-pcDNA3-VP1 intranasal immunization resulted in 42.9% protection of mice against lethal CVB3 challenge and a significant reduction of viral load after acute CVB3 infection. Meanwhile no myonecrosis or infiltrating immune cells indicating ongoing myocarditis was detected in hearts of surviving mice treated with chitosan-DNA. Together, Our data show that intranasal delivery of chitosan-DNA vaccine successfully induced mucosal SIgA secretion and might be a promising vaccine candidate to protect against CVB3 infection. © 2004 Elsevier Ltd. All rights reserved.
KeywordChitosan-DNA CVB3 Intranasal Mucosal SIgA Protection
DOI10.1016/j.vaccine.2004.03.033
URLView the original
Language英語
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Cited Times [WOS]:67   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Ctr. Gene Immunization Vacc. Res.
2.Fudan University Shanghai Medical College
Recommended Citation
GB/T 7714
Xu W.,Shen Y.,Jiang Z.,et al. Intranasal delivery of chitosan-DNA vaccine generates mucosal SIgA and anti-CVB3 protection[J]. Vaccine,2004,22(27-28):3603-3612.
APA Xu W.,Shen Y.,Jiang Z.,Wang Y.,Chu Y.,&Xiong S..(2004).Intranasal delivery of chitosan-DNA vaccine generates mucosal SIgA and anti-CVB3 protection.Vaccine,22(27-28),3603-3612.
MLA Xu W.,et al."Intranasal delivery of chitosan-DNA vaccine generates mucosal SIgA and anti-CVB3 protection".Vaccine 22.27-28(2004):3603-3612.
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