UM
Construction of an eukaryotic expression plasmid for short peptides used in DNA immunization
Xu H.B.; Xu W.; Chu Y.W.; Wang Y.; Zhang R.H.; Xiong S.D.
2005
Source PublicationXi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
ISSN10078738
Volume21Issue:1Pages:29-32
AbstractAIM: To construct a plasmid which efficiently express short peptides in DNA immunization. METHODS: The plasmid containing peptide-expressing cassette (PEC) was constructed and its effect in DNA immunization was investigated, using a DHBV B cell epitope as the short peptide. The peptide in vitro was detected by DOT-EIA. The BALB/c mice were immunized with the empty plasmid or the recombinant plasmid, and the specific antibodies against the epitope in the sera of the mice were determined by ELISA. RESULTS: The plasmid containing peptide-expressing cassette (PEC) was successfully constructed. Recombinant epitope-based plasmid could efficiently express the short peptide in vitro and induce immune response against it in DNA immunization. CONCLUSION: The constructed vector provides highly efficient short peptide expression in DNA immunization.
URLView the original
Language英語
Fulltext Access
Document TypeJournal article
CollectionUniversity of Macau
AffiliationFudan University Shanghai Medical College
Recommended Citation
GB/T 7714
Xu H.B.,Xu W.,Chu Y.W.,et al. Construction of an eukaryotic expression plasmid for short peptides used in DNA immunization[J]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology,2005,21(1):29-32.
APA Xu H.B.,Xu W.,Chu Y.W.,Wang Y.,Zhang R.H.,&Xiong S.D..(2005).Construction of an eukaryotic expression plasmid for short peptides used in DNA immunization.Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology,21(1),29-32.
MLA Xu H.B.,et al."Construction of an eukaryotic expression plasmid for short peptides used in DNA immunization".Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 21.1(2005):29-32.
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