UM
A point-of-need enzyme linked aptamer assay for Mycobacterium tuberculosis detection using a smartphone
Li, Lei; Liu, Zhonggang; Zhang, Heng; Yue, Wanqing; Li, Cheuk-Wing; Yi, Changqing
2018-01
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
Volume254Pages:337-346
AbstractDue to the poor sensitivities and specificities of antibody based point of need devices, we have developed a low-cost and reliable latent tuberculosis infection (LTBI) screening system based on enzyme linked aptamers which are cost effective and chemically stable. Specific recognition of mannose-capped lipoarabinomannan on the surface of Mycobacterium tuberculosis has been achieved for the development of direct and indirect dot-blot approaches which are designed for simultaneous quantitative analysis of multiple samples and identification of mycobacterial strains, respectively. Compared with traditional acid-fast staining assay used for LTBI screening, our direct dot-blot system has offered a lower limit of quantitation at 10(4) CFU/mL and higher accuracy. In contrast to traditional bacterial culture approach which takes 35 weeks, our assay can be completed within 5 h, which is a remarkable increase in assay efficiency. To maximize the portability and fulfill the needs for LTBI screening in developing countries, an Android application has been developed to carry out colorimetric analysis of images captured by a smartphone camera, followed by generating and sharing of diagnostic reports through internet connectivity. Upon promotion, we believe this low-cost and effective LTBI screening system will control the outspread of tuberculosis and save many lives, particularly in developing countries.
KeywordMycobacterium tuberculosis Enzyme linked aptamer assay Dot-blot assays Smartphone Android application
DOI10.1016/j.snb.2017.07.074
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000413308000042
PublisherELSEVIER SCIENCE SA
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:13   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Li, Lei,Liu, Zhonggang,Zhang, Heng,et al. A point-of-need enzyme linked aptamer assay for Mycobacterium tuberculosis detection using a smartphone[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,254:337-346.
APA Li, Lei,Liu, Zhonggang,Zhang, Heng,Yue, Wanqing,Li, Cheuk-Wing,&Yi, Changqing.(2018).A point-of-need enzyme linked aptamer assay for Mycobacterium tuberculosis detection using a smartphone.SENSORS AND ACTUATORS B-CHEMICAL,254,337-346.
MLA Li, Lei,et al."A point-of-need enzyme linked aptamer assay for Mycobacterium tuberculosis detection using a smartphone".SENSORS AND ACTUATORS B-CHEMICAL 254(2018):337-346.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Lei]'s Articles
[Liu, Zhonggang]'s Articles
[Zhang, Heng]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Lei]'s Articles
[Liu, Zhonggang]'s Articles
[Zhang, Heng]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Lei]'s Articles
[Liu, Zhonggang]'s Articles
[Zhang, Heng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.