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Th2-dominated antitumor immunity induced by DNA immunization with the genes coding for a basal core peptide PDTRP and GM-CSF
Chu Y.1; Xia M.1; Lin Y.1; Li A.1; Wang Y.1; Liu R.1; Xiong S.1
2006-05-01
Source PublicationCancer Gene Therapy
ISSN09291903 14765500
Volume13Issue:5Pages:510-519
AbstractOur previous study showed that DNA vaccination with a plasmid vector encoding a core peptide of mucin1 (PDTRP) provided modest protection against challenge with tumor cells that expressed mucin1 protein. We report here that a DNA vaccine comprising a modified PDTRP plasmid and GM-CSF coding sequence at the C-terminus induced better protection against tumor challenge. The increased protection was directly correlated with a stronger PDTRP-specific immune response induced by the GM-CSF fusion plasmid. The plasmid encoding GM-CSF and the target PDTRP antigen induced a greater PDTRP-specific Th proliferation, antibodies, and cytotoxicity. Interestingly, the modified plasmid vaccine predominantely enhanced the type 2 immune responses manifested by an increased IgG1 to IgG2a antibody ratio and a greater induction of GATA-3 and IL-4 mRNA than that of T-bet and IFN-γ mRNA in spleen cells from vaccinated mice. In addition, protection against tumor challenge in vaccinated mice showed that there was no significant change in mice survival after in vivo CD8 + CTL depletion, indicating that antitumor immunity augmented by plasmid encoding GM-CSF and target PDTRP gene vaccine was dominated by Th2 immune response. © 2006 Nature Publishing Group All rights reserved.
Keywordγ1neo-PDTRP/GM-CSF Gene immunization Mucin1 Th2 type
DOI10.1038/sj.cgt.7700913
URLView the original
Language英語
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Cited Times [WOS]:19   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Fudan University Shanghai Medical College
2.E-Institute of Shanghai Universities
Recommended Citation
GB/T 7714
Chu Y.,Xia M.,Lin Y.,et al. Th2-dominated antitumor immunity induced by DNA immunization with the genes coding for a basal core peptide PDTRP and GM-CSF[J]. Cancer Gene Therapy,2006,13(5):510-519.
APA Chu Y..,Xia M..,Lin Y..,Li A..,Wang Y..,...&Xiong S..(2006).Th2-dominated antitumor immunity induced by DNA immunization with the genes coding for a basal core peptide PDTRP and GM-CSF.Cancer Gene Therapy,13(5),510-519.
MLA Chu Y.,et al."Th2-dominated antitumor immunity induced by DNA immunization with the genes coding for a basal core peptide PDTRP and GM-CSF".Cancer Gene Therapy 13.5(2006):510-519.
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