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CD4+ CD25+ regulatory T cells exhibit cytotoxicity against dendritic cells
Ren X.-F.; Xiong S.-D.; Wang Y.
2006-11-30
Source PublicationChinese Journal of Microbiology and Immunology
ISSN02545101
Volume26Issue:11Pages:1017-1021
AbstractObjective: To investigate whether CD4 CD25 regulatory T cells could regulate dendritic cells. Methods: CD4 CD25 regulatory T cells were isolated by MACS(magnetic cell sorting) from naïve BALB/ c mice. Their suppression on the proliferation of CD4 CD25 T cells was analyzed by cell proliferation assay. Autologous DCs were prepared from bone marrow progenitors and cultured with GM-CSF/IL-4. The characters of these DCs were analyzed by FACS. CD4 CD25 regulatory T cells were activated by CD3/CD28 antibodies and their cytotoxic effects on autologous DCs were evaluated in vitro by flow-based killing assay. To analyze the role of the perforin/granzyme pathway in killing, EGTA was added to inhibit calcium-dependent perform polymerization. Results: It was found that DCs derived from bone marrow expressed CD11c, MHC II and low levels of other co-stimulatory molecules such as CD80, CD86. CD4 CD25 regulatory T cells could be successfully classified by MACS with 98% purity and specifically express the Foxp3 gene. CD4 CD25 regulatory T cells stimulated by CD3/ CD28 exhibited cytotoxic effects on autologous DC targets(P < 0.05), and the process could be partially inhibited by EGTA(P < 0.05). Conclusion: CD4 CD25 regulatory T cells could regulate immune response by killing autologous DCs, and perforin/granzyme pathway might participate in this process.
KeywordCD4+ CD25+ regulatory T cells Dendritic cells EGTA Killing
URLView the original
Language英語
Fulltext Access
Document TypeJournal article
CollectionUniversity of Macau
AffiliationFudan University
Recommended Citation
GB/T 7714
Ren X.-F.,Xiong S.-D.,Wang Y.. CD4+ CD25+ regulatory T cells exhibit cytotoxicity against dendritic cells[J]. Chinese Journal of Microbiology and Immunology,2006,26(11):1017-1021.
APA Ren X.-F.,Xiong S.-D.,&Wang Y..(2006).CD4+ CD25+ regulatory T cells exhibit cytotoxicity against dendritic cells.Chinese Journal of Microbiology and Immunology,26(11),1017-1021.
MLA Ren X.-F.,et al."CD4+ CD25+ regulatory T cells exhibit cytotoxicity against dendritic cells".Chinese Journal of Microbiology and Immunology 26.11(2006):1017-1021.
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